松果菊苷调节CXCL12/CXCR4信号通路对牙髓干细胞增殖、迁移和分化的影响  

Impacts of echinacetin on the proliferation,migration,and differentiation of dental pulp stem cells by regulating the CXCL12/CXCR4 signaling pathway

作　　者：宋新燕 赵阳 罗菲菲 吴亚磊 SONG Xinyan;ZHAO Yang;LUO Feifei;WU Yalei(Dentistry and Pulp Department 1,Handan Stomatological Hospital,Handan 056001,China)

机构地区：[1]邯郸市口腔医院牙体牙髓一科,河北邯郸056001

出　　处：《现代医学》2024年第11期1688-1695,共8页Modern Medical Journal

基　　金：邯郸市科学技术研究与发展计划项目(1528108079-4)。

摘　　要：目的:探讨松果菊苷(ECH)调节CXC趋化因子配体12(CXCL12)/CXC趋化因子受体4(CXCR4)信号通路对牙髓干细胞(DPSCs)增殖、迁移和分化的影响。方法:以DPSCs为研究对象,分别经不同浓度(0.01 mg·mL^(-1)、0.1 mg·mL^(-1)、1 mg·mL^(-1))的ECH处理,记为ECH低浓度(ECH-L)组、ECH中浓度(ECH-M)组、ECH高浓度(ECH-H)组;以1 mg·mL^(-1)ECH、100 nmol·L-1AMD3100同时处理DPSCs,记为ECH-H+AMD3100组,并以未经处理的DPSCs为空白对照组;MTT法检测细胞增殖;Transwell实验检测细胞迁移;Western blot检测CXCL12、CXCR4蛋白表达;成骨诱导21 d后,茜素红S染色评估矿化结节形成;碱性磷酸酶(ALP)试剂盒分析ALP活性;qRT-PCR检测牙本质基质蛋白1(DMP1)、骨钙素(OCN)、ALP mRNA表达水平。结果:与空白对照组相比,ECH-L组、ECH-M组、ECH-H组CXCL12、CXCR4蛋白表达、迁移数、ALP含量、矿化结节含量、A_(450)值、DMP1、OCN、ALP mRNA表达水平显著增加,不同浓度ECH间均具有统计学差异(P<0.05);与ECH-H组相比,ECH-H+AMD3100组CXCL12、CXCR4蛋白表达、迁移数、ALP含量、矿化结节含量、A_(450)值、DMP1、OCN、ALP mRNA表达水平显著降低(P<0.05)。结论:ECH通过上调CXCL12/CXCR4信号通路促进DPSCs增殖、迁移和分化。Objective:To investigate the impacts of Echinacetin(ECH)on the proliferation,migration,and differentiation of dental pulp stem cells(DPSCs)by regulating the CXC chemokine ligand 12(CXCL12)/CXCR4 signaling pathway.Methods:DPSCs were studied and subjected to different concentrations of ECH(0.01 mg·mL^(-1),0.1 mg·mL^(-1),1 mg·mL^(-1)),and they were grouped into low ECH concentration(ECH-L)group,medium ECH concentration(ECH-M)group,and high ECH concentration(ECH-H)group;DPSCs were treated with 1 mg·mL^(-1)ECH and 100 nmol·L-1 AMD3100 simultaneously,and recorded as the ECH-H+AMD3100 group,and untreated DPSCs were used as blank control group;MTT method was applied to detect cell proliferation;Transwell experiment was applied to detect cell migration;Western blot was applied to detect the expression of CXCL12 and CXCR4 proteins;21 days after osteogenic induction,alizarin red S staining was applied to evaluate the formation of mineralized nodules;alkaline phosphatase(ALP)assay kit was applied to analyze ALP activity;qRT-PCR was applied to detect the expression levels of dentin matrix protein 1(DMP1),osteocalcin(OCN),and ALP mRNA.Results:Compared with the blank control group,the expression of CXCL12,CXCR4 protein,migration number,ALP content,mineralized nodule content,A_(450)value,expression levels of DMP1,OCN,and ALP mRNA in the ECH-L group,ECH-M group,and ECH-H group obviously increased,there were statistical differences among different concentrations of ECH(P<0.05);compared with the ECH-H group,the expression of CXCL12,CXCR4 protein,migration number,ALP content,mineralized nodule content,A_(450)value,expression levels of DMP1,OCN,and ALP mRNA in the ECH-H+AMD3100 group obviously decreased(P<0.05).Conclusion:ECH promotes the proliferation,migration,and differentiation of DPSCs by up-regulating the CXCL12/CXCR4 signaling pathway.

关 键 词：松果菊苷 CXCL12/CXCR4 牙髓干细胞 增殖 迁移 分化 

分 类 号：R781.3[医药卫生—口腔医学]