金黄地鼠不同病理状态颊囊黏膜的近红外荧光成像差异分析  

Differences in near-infrared fluorescence imaging and histological analysis of cheek mucosa in golden hamsters with different pathological states

作　　者：谢狄亚 单丹妮 张磊[1] 陈盛[1] 那潆予 王志勇[1] Xie Diya;Shan Danni;Zhang Lei;Chen Sheng;Na Yingyu;Wang Zhiyong(Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Research Institute of Stomatology,Nanjing University,Nanjing 210008,China;Dept.of Stomatology,Nanjing Honghua Community Health Service Center,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院,南京市口腔医院,南京大学口腔医学研究所,南京210008 [2]南京红花社区卫生服务中心口腔科,南京210008

出　　处：《华西口腔医学杂志》2024年第6期716-722,共7页West China Journal of Stomatology

基　　金：江苏省重点研发计划——社会发展项目(BE2018618);南京市医学科技发展项目(YKK19089)。

摘　　要：目的探讨分析吲哚菁绿(ICG)近红外荧光(NIF)成像技术对口腔潜在恶性疾患及口腔鳞状细胞癌早期诊断的可行性。方法利用二甲基苯并蒽丙酮溶液,给予金黄地鼠颊囊黏膜不同时间的局部刺激,诱导建立轻/中度异常增生、重度异常增生、鳞状细胞癌等病理状态的颊黏膜病变模型。采用ICG-NIF技术对病变组织进行荧光信号定量分析,免疫组织化学染色法对不同病理状态黏膜的微血管密度(MVD)、微淋巴管密度(MLVD)进行分析。结果ICG-NIF荧光定量分析结果显示,实验组中黏膜病变组织的荧光强度均高于正常黏膜,差异具有统计学意义(P<0.05),且实验组中黏膜病变恶性程度越高,其荧光强度越高。组织学分析显示,随着黏膜病变恶性程度增加,MVD增加(P<0.05),MLVD减小(P<0.05)。结论金黄地鼠异常增生的颊部黏膜病变与正常黏膜组织间具有ICG-NIF荧光信号差异,荧光定量分析方法具有辅助鉴别的临床应用潜能。Objective To explore and analyze the feasibility of using indocyanine green(ICG)near-infrared fluorescence(NIF)imaging technology for the early diagnosis of oral potential malignant disorders and oral squamous cell carcinoma.Methods 7,12-Dimethylbenz[a]anthracene in acetone solution was used to induce various pathological models of buccal mucosal lesions(mild/moderate dysplasia,severe dysplasia,squamous cell carcinoma)in golden hamster.ICG-NIF was conducted for the quantitative analysis of the fluorescence signal of lesion tissue,and evaluation of the diagnostic and discriminative capabilities of the ICG-NIF technology for mucosal lesions in various pathological states.Immunohistochemical staining was perform to examine the microvessel density(MVD)and microlymphatic vessel density(MLVD)of mucosa in various pathological states and explore the histological reasons underlying the differences in fluorescence signals.Results The results of ICG-NIF fluorescence quantitative analysis reveal the higher fluorescence intensity of mucosal lesions in the experimental group compared with that of the normal mucosa on the control side,with statistical differences(P<0.05).Moreover,the more severe the malignancy of mucosal lesions in the experimental group,the higher the fluorescence intensity.According to histopathological analysis,the malignant progression of mucosal lesions in golden hamsters was accompanied with an increase in MVD(P<0.05)and a decrease in MLVD(P<0.05).Conclusion The abnormal proliferation of mucosal lesions in golden hamsters exhibits a difference in ICG-NIF fluorescence signal compared with normal mucosal tissue.Fluorescence quantitative analysis methods can provide assistance in differentiation and show potential for clinical applications.

关 键 词：吲哚菁绿 近红外荧光成像 口腔潜在恶性疾患 口腔鳞状细胞癌 

分 类 号：R780.2[医药卫生—口腔医学]