栉孔扇贝STARD3 cDNA序列分析及表达模式研究  

Sequence analysis and expression pattern of STARD3 cDNA in Chlamys farreri

作　　者：罗冬艳 翟晓桐 刘晓玲[1] 崔龙波[1] LUO Dongyan;ZHAI Xiaotong;LIU Xiaoling;CUI Longbo(College of Life Sciences,Yantai University,Yantai Shandong 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《海洋渔业》2024年第5期579-585,共7页Marine Fisheries

基　　金：山东省贝类产业技术体系建设专项资金(SDAIT-14-05);山东省自然科学基金(ZR2022MC126)。

摘　　要：对栉孔扇贝(Chlamys farreri)STARD3基因序列特征及表达模式进行了分析。结果显示,该基因的编码序列(coding sequence,CDS)长1512 bp,编码503个氨基酸,含有START和MENTAL 2个主要结构域及FFAT基序,与其他物种一致。采用荧光定量PCR(qRT-PCR)检测了STARD3在栉孔扇贝不同组织及性腺发育周期的表达,结果显示,STARD3基因在两性性腺中的表达量显著高于其在其他组织中的表达量(P<0.05),在肾和鳃中也有较高表达;STARD3基因在性腺发育各期中均有表达,其在增殖期的表达量高于其他两个时期,表明STARD3在栉孔扇贝性腺发育早期发挥主要作用,但其生物学功能还有待深入研究。In this paper,the sequence and expression characteristics of STARD3 in Chlamys farreri were studied to provide a basis for further comprehensive exploration of its gonadal development mechanism.STARD3 is a member of the steroidogenic acute regulatory protein related lipid transfer domain(START)family.This study using the existing transcriptome data of Chlamys farreri,obtained the CDS sequence of STARD3,analyzed the amino acid sequence of STARD3 using DNAMAN,compared its protein homology,and finally constructed the phylogenetic tree using neighbor joining(NJ)method.At the same time,RNA was extracted in different tissues(kidney,gill,mantle,adductor muscle,hepatopancreas,testis and ovary)and different stages(proliferation stage,growth stage and mature stage)of Chlamys farreri,and then was reverse transcribed into cDNA,as a template to detect the relative expression of the gene in different tissues and different stages.The results showed that the coding sequence(CDS)of the gene was 1512 bp,encoding 503 amino acids.The amino acid sequence comparison of different species showed that the STARD3 of Chlamys farreri had high homology with other species,up to 92.01%,which included START domain,MLN64 Net alterminal(METAL)domain and diphenylalanine(FF)-acid orbital(AT)(FFAT)motif,as well as the amino acid residues required for biological functions,such as Arg,Ser,Phe,Leu and Tyr.Phylogenetic analysis showed that STARD3 protein sequence of Chlamys farreri first clustered with scallops such as Mizuhopecten yessoensis,followed by Mytilus edulis and Mytilus coruscus,next followed by Sepia pharaonis and Octopus bimaculoides belonging to the same mollusca phyla,then clustered with lower vertebrates,and finally with higher mammals,which proved that the phylogenetic relationship of STARD3 in Chlamys farreri was consistent with the taxonomic position of the species.The results of qRTet alPCR in kidney,hepatopancreas,mantle,adductor muscle,gill,ovary and testis showed that STARD3 was detected in the above organizations,indicating that

关 键 词：栉孔扇贝 STARD3基因 表达横式 

分 类 号：S917.4[农业科学—水产科学]