液相色谱-质谱非靶向代谢组学分析3种不同生产原料波杂发酵饮品的代谢差异  

Liquid Chromatography-Mass Spectrometry-Based Untargeted Metabolomics Analysis of Differential Metabolites in Three Types of Boza Made from Different Raw Materials

作　　者：汪琪 高雁[1] 薛娟娟 曾军[1] 霍向东[1] 赵仲凯 WANG Qi;GAO Yan;XUE Juanjuan;ZENG Jun;HUO Xiangdong;ZHAO Zhongkai(Xinjiang Laboratory of Special Environmental Microbiology,Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Ürümqi 830091,China;College of Life Science and Technology,Xinjiang University,Ürümqi 830046,China)

机构地区：[1]新疆农业科学院微生物应用研究所,新疆特殊环境微生物实验室,新疆乌鲁木齐830091 [2]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《食品科学》2024年第23期81-92,共12页Food Science

基　　金：新疆维吾尔自治区奶产业技术体系项目(NCYJSTX2024);农业科技创新稳定支持项目(xjnkywdzc-2023005-6)。

摘　　要：利用超高效液相色谱-串联飞行时间质谱对3种不同生产原料的波杂发酵饮品进行非靶向代谢组学研究。采用主成分分析、偏最小二乘判别分析等多元统计分析方法,筛选出差异代谢物,并对差异代谢物及其通路富集情况进行研究。结果表明,3种不同生产原料的波杂样品组间代谢物均存在明显差异。非遗波杂(G1)、传统塔尔米波杂(G2)和传统玉米波杂(G3)组间筛选的显著差异代谢物分别是109(G1 vs G2)、124种(G1 vs G3)和114种(G3 vs G2)。这些差异代谢物主要体现在脂质和类脂分子、有机酸及其衍生物、有机杂环类化合物、苯丙烷类和聚酮类化合物等,差异可能是由原料的不同所引起。京都基因与基因组百科全书代谢通路富集分析显示,这些差异代谢物主要参与了多种植物次生代谢产物的生物合成、苯丙烷类化合物的生物合成、黄酮类物质的生物合成及其降解等代谢通路。本研究探索了3种不同生产原料的波杂发酵饮品的差异代谢物及代谢通路,以期更好地为波杂发酵饮品综合开发利用提供科学理论依据。Untargeted metabolomics based on ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS)was adopted to study three types of Boza,made from different raw materials.Multivariate statistical techniques such as principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)were used to screening for differential metabolites,and pathway enrichment analysis of differential metabolites was carried out.The results showed that there were significant differences in metabolites among the three types of Boza.Altogether,109,124 and 114 differential metabolites were identified in intangible cultural heritage(G1)vs traditional Talmi Boza(G2),G1 vs traditional corn Boza(G3),and G3 vs G2,respectively.These differential metabolites mainly included lipids and lipidlike molecules,organic acids and their derivatives,organic heterocyclic compounds,phenylpropanoids,and polyketones.The difference might be caused by the difference in raw materials.According to Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathway enrichment analysis,these differential metabolites mainly participated in the biosynthesis of various secondary metabolites in plants,the biosynthesis of phenylpropanoids,the biosynthesis and degradation of flavonoids,and other metabolic pathways.This study provides a theoretical basis for the comprehensive development and utilization of Boza.

关 键 词：波杂 液相色谱-质谱法 非靶向代谢组学 代谢物 代谢通路 

分 类 号：Q93[生物学—微生物学]