双信标荧光生物传感器同时检测黄曲霉毒素及其产毒基因  

Dual-beacon fluorescent biosensor for simultaneous detection of aflatoxin and its toxin-producing genes

作　　者：李雅琪 于新 彭双凤 孔德昭 刘畅 史巧巧 陈勇[1] LI Yaqi;YU Xin;PENG Shuangfeng;KONG Dezhao;LIU Chang;SHI Qiaoqiao;CHEN Yong(School of Environmental Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,China;School of Grain Science and Technology,Jiangsu University of Science and Technology,Zhenjiang 212100,China;School of Environmental and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang 212100,China;Advanced Technology Institute of Suzhou,Suzhou 215123,China)

机构地区：[1]华中科技大学环境科学与工程学院,武汉430074 [2]江苏科技大学粮食学院,镇江212100 [3]江苏科技大学环境与化学工程学院,镇江212100 [4]苏州中科先进技术研究院有限公司,苏州215123

出　　处：《分析试验室》2024年第11期1586-1593,共8页Chinese Journal of Analysis Laboratory

基　　金：中国博士后基金面上项目(2021M692370)资助。

摘　　要：构建了基于硅掺杂碳点(Si-CDs)和锰掺杂硫化锌(Mn:Zn S)双量子点信标与黑洞猝灭剂(BHQ)之间基于荧光共振能量转移(FRET)的荧光生物传感器,采用适配体/DNA互补配对原则同时特异性识别目标物黄曲霉毒素B1(AFB_(1))及其霉菌产毒基因(aflD)。该方法在10~300 nmol/L浓度范围内对AFB_(1)和aflD的浓度呈良好的线性关系,对AFB_(1)的检测相关系数(R^(2))为0.9928,检出限(LOD)为2.83 nmol/L;对aflD的R^(2)为0.9967,LOD为2.67 nmol/L。在多种真菌毒素和干扰DNA片段存在下,该传感器能够准确识别目标物AFB_(1)和aflD,展现了良好的选择性和重现性,并成功应用于实际玉米样品的检测。双目标的同时检测,有效提升了检测效率,为AFB_(1)及aflD的早期监测提供了依据。A fluorescent biosensor was constructed to recognize the target aflatoxin B1 (AFB1) and its mycobacterial toxin-producing genes (aflD) simultaneously and specifically.It employed the principle of aptamer/DNA complementary pairing based on Forster resonance energy transfer (FRET) between dual beacons of silica dopped carbon dots (Si-CDs) and Mn∶Zn S quantum dots (QDs) and black hole quencher (BHQ).The method showed good linearity in the concentration range of 10-300 nmol/L for AFB1 and aflD,with correlation coefficient (R^(2)) of 0.9928 and limit of detection (LOD) of 2.83 nmol/L for AFB1,and R^(2) of 0.9967 and LOD of 2.67 nmol/L for aflD.In the presence of multiple mycotoxins and interfering DNA fragments,this sensor could accurately recognize the targets AFB1 and aflD,demonstrating good selectivity and reproducibility,and it was successfully applied to the monitoring of actual corn samples.The simultaneous detection of dual targets effectively improves the detection efficiency and provides a basis for the early monitoring of aflatoxin B1 and its mycotoxin-producing genes.

关 键 词：量子点 荧光生物传感器 黄曲霉毒素 aflD基因 同时检测 

分 类 号：O657.1[理学—分析化学]