液相色谱-气相色谱质谱联用法分析氯氰菊酯农药手性异构体  

Analysis of chiral isomers of cypermethrin pesticide by liquid chromatography-gas chromatography-mass spectrometry

作　　者：戚燕 张楠[1] 赵旭东[1] 刘平[1,2] 赵榕 范赛[1,2] QI Yan;ZHANG Nan;ZHAO Xudong;LIU Ping;ZHAO Rong;FAN Sai(Beijing Center for Disease Prevention and Control,Beijing 100013,China;School of Public Health,Capital Medical University,Beijing 100069,China;不详)

机构地区：[1]北京市疾病预防控制中心,100013 [2]首都医科大学公共卫生学院

出　　处：《首都公共卫生》2024年第4期217-221,共5页Capital Journal of Public Health

基　　金：国家自然科学基金青年基金项目(编号:81302431)。

摘　　要：目的建立氯氰菊酯的8种对映异构体手性拆分方法。方法采用液相色谱-气相色谱-质谱联用法,通过Chiralcel OD-3[纤维素-三(3,5-二甲基苯基氨基甲酸酯)]手性柱,得到8种氯氰菊酯异构体的7个色谱峰,分为两组分析。采用液相色谱-气相色谱-质谱联用系统,将两个分离组分别通过进样针模式的LC-GC接口在线注入PTV进样口中实现浓缩,再使用气相色谱串联质谱方法进行异构体的完全分离。结果实现了氯氰菊酯8个对映异构体的完全分离。结论开发的液相色谱-气相色谱-质谱联用方法能够实现氯氰菊酯8种异构体的完全拆分,缩短了分析时间,提高了分离效率。Objective This study aims to establish an effective chiral separation method for eight enantiomers of cypermethrin.Methods A liquid chromatography-gas chromatography-mass spectrometry(LC-GC-MS)approach was utilized to achieve separation,employing a Chiralcel OD-3 chiral column.Through this method,seven chromatographic peaks corresponding to chiral cypermethrin were obtained and subsequently categorized into two groups.The LC-GC-MS system was then employed to concentrate these two groups by utilizing an online injection into the PTV injection port via the LC-GC interface in injection needle mode.Complete separation of the isomers was achieved using GC-MS/MS.Results Complete separation of all eight enantiomers of cypermethrin was successfully achieved.Conclusions The developed LC-GC-MS method enables the complete separation of the eight isomers of cypermethrin,thereby reducing analysis time and enhancing separation efficiency.

关 键 词：氯氰菊酯 手性拆分 液相色谱 气相色谱串联质谱 

分 类 号：O657.7[理学—分析化学] X592[理学—化学]