基于生物信息学筛选食管鳞状细胞癌免疫浸润相关基因  

Screening of immune infiltration related genes in esophageal squamous cell carcinoma based on bioinformatics

作　　者：闫煜钰 彭昊[2] 于子恒 何建伟[1] YAN Yuyu;PENG Hao;YU Ziheng;HE Jianwei(Laboratory Center,the First Affiliated Hospital of Shihezi University,Shihezi 832008;Pathology Department,Shihezi University School of Medicine,Shihezi 832002,China)

机构地区：[1]石河子大学第一附属医院检验中心,新疆石河子832008 [2]石河子大学医学院病理系,新疆石河子832002

出　　处：《临床医学研究与实践》2024年第33期1-4,共4页Clinical Research and Practice

基　　金：国家自然科学基金资助项目(No.82260556);石河子大学医学院第一附属医院科研项目(No.BS202105)。

摘　　要：目的利用生物信息学方法筛选食管鳞状细胞癌(ESCC)免疫浸润相关基因。方法利用基因表达综合数据库(GEO)获取ESCC相关数据集,筛选出差异表达基因(DEGs)与免疫相关基因(IRGs)之间差异表达的免疫相关基因(DEIRGs),并对DEIRGs进行富集分析及绘制蛋白质相互作用(PPI)网络图。采用CIBERSORT和ESTIMATE方法分析ESCC免疫细胞浸润情况。结果共筛选出53个DEIRGs。CXCL8在PPI网络中排名第一。ESTIMATE分析结果显示,ESCC组中基质、免疫评分更高;CIBERSORT分析结果显示,CD8 T细胞在ESCC组中浸润水平较低。Spearman相关性分析结果显示,CXCL8基因与CD8 T细胞呈负相关。结论CXCL8是免疫浸润相关的关键基因,其与ESCC的发生发展密切相关。Objective The immune infiltration related genes of esophageal squamous cell carcinoma(ESCC)were screened by bioinformatics methods.Methods The gene expression omnibus(GEO)was used to obtain ESCC-related data sets,the different expression immune-related genes(DEIRGs)between differential expression genes(DEGs)and immune-related genes(IRGs)were screened out,and DEIRGs were subjected to enrichment analysis and protein-protein interaction(PPI)network diagram was drawn.CIBERSORT and ESTIMATE methods were used to analyze the infiltration of immune cells in ESCC.Results A total of 53 DEIRGs were screened.CXCL8 ranked first in the PPI network.The results of ESTIMATE analysis showed that the matrix and immune scores in the the ESCC group were higher;and the results of CIBERSORT analysis showed that the infiltration level of CD8 T cells was lower in the ESCC group.Spearman correlation analysis results showed that CXCL8 gene was negatively correlated with CD8 T cells.Conclusion CXCL8 is a key gene related to immune infiltration,which is closely related to the occurrence and development of ESCC.

关 键 词：生物信息学 食管鳞状细胞癌 CXCL8 免疫浸润 

分 类 号：R734[医药卫生—肿瘤]