Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment  

作　　者：Weiqin Sheng Ying Li Chunlian Qin Zhonghai Zhang Yuxiang Pan Zhicheng Tong Chong Teng Xinwei Wei 

机构地区：[1]School of Electronic Information,Hangzhou Dianzi University,Hangzhou 310018,China [2]College of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310058,China [3]ZJU-Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University,Hangzhou 311200,China [4]Department of Orthopedics,The Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu 322000,China [5]Key Laboratory of Advanced Drug Delivery Systems of Zhejiang Province,College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China 123

出　　处：《Bio-Design and Manufacturing》2024年第6期972-982,共11页生物设计与制造（英文）

基　　金：supported by the Zhejiang Provincial Natural Science Foundation of China(No.LQ23E010004);the National Key Research and Development Program of China(No.2021YFB3200801)。

摘　　要：Intracellular electrophysiological research is vital for biological and medical research.Traditional planar microelectrode arrays(MEAs)have disadvantages in recording intracellular action potentials due to the loose cell-electrode interface.To investigate intracellular electrophysiological signals with high sensitivity,electroporation was used to obtain intracellular recordings.In this study,a biosensing system based on a nanoporous electrode array(NPEA)integrating electrical perforation and signal acquisition was established to dynamically and sensitively record the intracellular potential of cardiomyocytes over a long period of time.Moreover,nanoporous electrodes can induce the protrusion of cell membranes and enhance cell-electrode interfacial coupling,thereby facilitating effective electroporation.Electrophysiological signals over the entire recording process can be quantitatively and segmentally analyzed according to the signal changes,which can equivalently reflect the dynamic evolution of the electroporated cardiomyocyte membrane.We believe that the low-cost and high-performance nanoporous biosensing platform suggested in this study can dynamically record intracellular action potential,evaluate cardiomyocyte electroporation,and provide a new strategy for investigating cardiology pharmacological science.

关 键 词：Nanoporous electrode array(NPEA) ELECTROPORATION Intracellular potential recording CARDIOMYOCYTE Membrane resealing 

分 类 号：R318[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学] TP212[一般工业技术—材料科学与工程]