EZ Prep清洗液替代二甲苯在眼组织特殊染色中的应用  

Application of EZ Prep cleaning solution as a xylene substitute in special staining of ocular tissues

作　　者：高欢欢[1] 林健贤[1] 陈淑霞 张文忻[1] 唐丽娟[1] 张平[1] GAO Huanhuan;LIN Jianxian;CHEN Shuxia;ZHANG Wenxin;TANG Lijuan;ZHANG Ping(State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangdong Provincial Key Laboratory of Ophthalmology Visual Science,Guangzhou 510060,China)

机构地区：[1]中山大学中山眼科中心,眼病防治全国重点实验室,广东省眼科视觉科学重点实验室,广州510060

出　　处：《眼科学报》2024年第11期571-577,共7页Eye Science

摘　　要：目的:探讨EZ Prep清洗液替代二甲苯进行手工脱蜡制作特殊染色片的效果。方法:应用EZ Prep清洗液替代二甲苯脱蜡,选取中山大学中山眼科中心临床病理科石蜡标本100例进行回顾性研究,常规切片后切片一式两份,分别采用传统二甲苯和EZ Prep清洗液手工脱蜡后按实验室标准化操作流程进行特殊染色,并比较脱蜡时间和染色效果。结果:EZ Prep清洗液手工脱蜡处理的切片在革兰染色(Gram's)、六胺银染色(GMS)、过碘酸-雪夫染色(PAS)、马松(Masson)三色染色、刚果红等多种特殊染色中的染色质量和二甲苯脱蜡处理的效果一致,两组间染色片优良率比较差异无统计学意义(Z=0.32,P>0.05),且平均脱蜡时间由(33.0±2.7)min缩短至(7.2±1.1)min,加快了染色出片时间。结论:EZ Prep清洗液可以替代二甲苯在眼组织特殊染色中脱蜡,并具有脱蜡时间短、环保的优点,值得推广应用。Objective:To investigate the effect of EZ Prep cleaning solution as an alternative to xylene for manual deparaffinization in the preparation of special staining slides.Methods:EZ Prep cleaning solution was utilized to replace xylene for deparaffinization in a retrospective study involving 100 paraffin-embedded specimens from the Clinical Pathology Department of Zhongshan Ophthalmic Center,Sun Yat-sen University.Routine sections were prepared and duplicated,with one set deparaffinized using traditional xylene and the other using EZ Prep cleaning solution.Subsequent special staining was performed following standardized laboratory protocols.Deparaffinization time and staining outcomes were compared.Results:Slides treated with EZ Prep cleaning solution for manual deparaffinization demonstrated staining quality comparable to xylene treatment across various special stains,including Gram's,GMS,PAS,Masson's trichrome,and Congo red.The difference in the excellent rate between the two methods is not statistically significant(χ2=0.33,P>0.05).Moreover,the average deparaffinization time is significantly reduced from 33.0±2.7 minutes to 7.2±1.1 minutes,thereby speeding up the staining process.Conclusion:EZ Prep could replace xylene deparaffinized sections in special staining of ocular tissues with the advantages of shorter deparaffinized time and environmental protection,which is worthy of promotion and application.

关 键 词：脱蜡 二甲苯 EZ Prep 特殊染色 

分 类 号：R365[医药卫生—病理学] TQ619.1[医药卫生—基础医学]