生防链霉菌17-7菌株发酵配方优化及发酵滤液稳定性研究  

Optimization of Fermentation Formula and Stability of Cultural Filtrate Produced by Streptomyces samsunensi 17-7

作　　者：刘一贤[1] 施玉萍[1] 李岚岚 戴利铭 蔡志英[1] LIU Yixian;SHI Yuping;LI Lanlan;DAI Liming;CAI Zhiying(Yunnan Key Laboratory of Sustainable Utilization Research on Rubber Tree/Yunnan Institute of Tropical Crops,Jinghong,Yunnan 666100,China)

机构地区：[1]云南省天然橡胶可持续利用研究重点实验室/云南省热带作物科学研究所,云南景洪666100

出　　处：《热带作物学报》2024年第12期2689-2696,共8页Chinese Journal of Tropical Crops

基　　金：云南省热带作物科学研究所青年成长基金项目(No.QNCZ2021-2,No.QNCZ2022-3);云南省热带作物科技创新专项资金项目(No.RF2024-4-1)。

摘　　要：为了提升链霉菌17-7菌株的抑菌活性,合理利用菌株的代谢产物,本研究以橡胶树褐根病菌为靶标菌,采用正交试验和单因子试验相结合,在原培养基的基础上对链霉菌17-7菌株的发酵培养基配方进一步优化,获得该菌株最适宜产活性物质的配方为:大豆粉25 g/L、玉米粉10 g/L、葡萄糖10 g/L、酵母粉2 g/L、K_(2)HPO_(4)1 g/L、NaCl 0.5 g/L、CaCO_(3)0.5 g/L。发酵滤液稀释500倍后对橡胶树褐根病菌的相对抑菌活性为51.37%,较优化前提高了17.66%,并且具有广谱的抑菌活性。在此基础上测定了温度、紫外线辐射、蛋白酶、酸碱度和贮存条件对17-7菌株发酵滤液抗真菌物质稳定性的影响。结果表明:17-7菌株发酵滤液对高温不敏感;紫外线照射1 h后发酵滤液的抑菌活性开始显著下降,长时间紫外照射并不会丧失抑菌活性;17-7菌株发酵滤液还具有较好的酸碱耐受性,pH为3~10时其发酵滤液均能发挥较好的抑菌活性;并且对胰蛋白酶和胃蛋白酶不敏感;菌株发酵滤液在低温(4℃)和室温(25℃)条件下贮存120 d仍能保持较强生物活性。对17-7菌株无菌发酵滤液稳定性和贮存条件的研究可为后续该菌株的开发利用提供理论依据和数据支持。In order to enhance the antifungal activity of Streptomyces samsunensis 17-7 strain and effectively utilize its secondary metabolites,this study focused on optimizing the fermentation medium formula.Orthogonal and single factor experiments were conducted based on the original medium,with Phellinus noxious as the indicator.The optimal formu-lation for producing active substances from the strain was determined to include 25 g/L soy powder,10 g/L corn meal,10 g/L glucose,2 g/L yeast extract,1 g/L K_(2)HPO_(4),0.5 g/L NaCl,and 0.5 g/L CaCO_(3).Following a 500 times dilution,the relative antifungal activity of the fermentation solution increased to 51.37%,a 17.66%improvement compared to the previous optimization,demonstrating a broad spectrum of antifungal activity.The stability of the antifungal substance was evaluated in response to various conditions such as temperature,pH,protease,UV-irradiation,and storage.Results indicated that the fermentation broth of strain 17-7 exhibited high temperature resistance,a significant decrease in anti-fungal activity after one hour of UV irradiation,but retained its activity after long-term exposure to UV light.Further-more,the broth demonstrated good acid-base tolerance,effective antibacterial activity in the pH range of 3-10,and re-sistance to trypsin and pepsin.The fermentation broth of strain 17-7 maintained its antifungal activity for 120 days at both 4℃and room temperature(25℃).This study on the stability and storage conditions of strain 17-7’s fermentation broth offers valuable data for potential future applications of the strain as a biocontrol agent.

关 键 词：链霉菌17-7 发酵配方优化 稳定性 抑菌活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]