辛烯基琥珀酸木薯淀粉钠无醇酯化制备与表征  

Preparation and Characterization of Tapioca Starch Sodium Octenyl Succinate with Non-alcohol Esterification

作　　者：高媛媛 李如一 李莹莹 周伟[1] 黄晓兵[1] 彭芍丹[1] 李积华[1,2] GAO Yuanyuan;LI Ruyi;LI Yingying;ZHOU Wei;HUANG Xiaobing;PENG Shaodan;LI Jihua(Agricultural Products Processing Research Institute/Key Laboratory of Tropical Crop Products Processing,Ministry of Agricul-ture and Rural Affairs,Zhanjiang,Guangdong 524001,China;Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 524091,China)

机构地区：[1]中国热带农业科学院农产品加工研究所/农业农村部热带作物产品加工重点实验室,广东湛江524001 [2]中国热带农业科学院,海南海口524091

出　　处：《热带作物学报》2024年第12期2719-2727,共9页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.321QN0925);海南省重点研发计划项目(No.ZDYF2023XDNY073);湛江市创新创业团队引育“领航计划”项目(No.211207157080998)。

摘　　要：辛烯基琥珀酸淀粉钠(starch sodium octenyl succinate,SSOS)是以淀粉与辛烯基琥珀酸酐(octenyl succinic anhydride,OSA)酯化制得的食品添加剂。本研究采用OSA乳化法替代有机试剂分散OSA对木薯淀粉进行酯化制备辛烯基琥珀酸木薯淀粉钠(tapioca starch sodium octenyl succinate,TSSOS)并对其进行表征。结果表明:大豆卵磷脂(soybean phospholipid,SP)为乳化剂制备的OSA乳液体系具有较好的稳定性和分散性,SP添加量为1.00%制备的SSOS(1.00%SP-SSOS)比异丙醇分散OSA制备的SSOS(IPA-SSOP)具有更高的辛烯基琥珀酸基团(OS基团)含量、取代度(DS)以及取代效率(SE)。傅里叶红外光谱显示,1.00%SP-SSOS和IPA-SSOS均在1572 cm^(-1)和1726 cm^(-1)处出现了2个新的特征峰,其中1.00%SP-SSOS的特征峰强度更强。激光共聚焦扫描显微镜分析也证实了荧光标记的OS基团在1.00%SP-SSOS中荧光强度略强,少部分淀粉颗粒内部显示亦有OS基团,这说明利用高压均质以及SP乳化剂将OSA分散为微米级乳液再进行酯化,可以提高OSA与淀粉的接触面积从而提高酯化反应效率。综上所述,采用OSA乳化法替代有机试剂分散OSA制备SSOS的方法可行,本研究为无醇酯化反应体系应用在SSOS大规模工业化生产中提供参考依据。Starch sodium octenyl succinate is a food additive produced by the process of esterification using starch and octenyl succinic anhydride.In this study,the tapioca starch sodium octenyl succinate was prepared and characterized with non-alcohol esterification by substituting the organic reagent-dispersed OSA with the OSA emulsification technique.The re-sults showed that OSA emulsion prepared with soybean phospholipid(SP)as an emulsifier had good stability and dispersibility.The octenyl succinic acid group content,degree of substitution,and substitution efficiency of 1.00%SP-SSOS made using OSA emulsions with 1.00%SP emulsifiers were found to be greater compared to IPA-SSOS made using isopropa-nol-dispersed OSA.FT-IR showed that 1.00%SP-SSOS and IPA-SSOS had two new characteristic peaks at 1572 cm^(-1)and 1726 cm^(-1),with 1.00%SP-SSOS having a stronger peak intensity.Confocal laser scanning microscopy confirmed that the fluorescence intensity of the fluorescence-labeled OS group was slightly stronger in 1.00%SP-SSOS,and a few starch particles contained OS groups inside.The results indicated that the use of high-pressure homogenization and emulsifiers to disperse OSA into micron-sized oily droplets for esterification could increase the contact area between OSA and starch,improving the efficiency of the esterification reaction.To summarize,it is possible to produce SSOS by utilizing the OSA emulsification technique as an alternative to organic reagents for dispersing OSA.This study would provide a reference for the application of an alcohol-free esterification reaction system in the large-scale industrial production of SSOS.

关 键 词：木薯淀粉 无醇酯化 辛烯基琥珀酸淀粉钠 取代度 激光共聚焦扫描显微镜 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]