枸杞多糖对大鼠视神经损伤后视网膜神经节细胞的保护作用  

Effect of Lycium barbarum polysaccharides on retinal ganglion cells after optic nerve injury in rats

作　　者：胡瑾 朱德军[1] 徐曼云 邹文青[1] HU Jin;ZHU Dejun;XU Manyun;ZOU Wenqing(Department of Ophthalmology,People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China)

机构地区：[1]宁夏回族自治区人民医院眼科,宁夏银川750002

出　　处：《宁夏医学杂志》2024年第11期929-932,I0001,共5页Ningxia Medical Journal

基　　金：宁夏重点研发计划项目(2021BEG03110)。

摘　　要：目的 观察枸杞多糖(LBP)对大鼠视神经损伤后视网膜神经节细胞(RGCs)的保护作用。方法 将48只健康雄性SD大鼠随机分为正常组(A组)、损伤组(B组),LBP治疗组(C组)和LBP预防组(D组)4组,每组12只。除A组外,其余3组均用微型视神经夹制备大鼠左眼视神经损伤模型。D组于造模前以LBP100 mg/(kg·d^(-1))灌胃2周,造模后灌胃3个月。B组和C组造模后每天分别以生理盐水和LBP 100 mg/(kg·d^(-1))灌胃3个月。处死前1周用荧光金(FG)注入双上丘标记RGCs。灌胃3个月后摘除大鼠左侧眼球,剥离视网膜铺片,计算RGCs密度,检测视网膜中超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果 A组、B组、C组和D组RGCs密度分别为(2 354±250)、(802±82)、(1 138±96)、(1 196±82)个/mm^(2),4组间比较差异有统计学意义(P<0.05);B组RGCs密度降低、大小形态不一、显色弱且排列不规则。A组、B组、C组和D组SOD含量分别为(77.93±9.64)、(24.23±7.44)、(40.47±16.94)、(42.15±8.87)U/mgprot, 4组间比较差异有统计学意义(P<0.05)。A组、B组、C组和D组MDA含量分别为(6.21±1.97)、(43.09±13.15)、(29.55±4.51)、(28.91±6.74)nmol/mgprot, 4组间比较差异有统计学意义(P<0.05)。结论 LBP通过上调外伤性视神经病变(TON)后RGCs中SOD含量,增强清除活性氧(ROS)的能力,抑制脂质过氧化反应,下调MDA的含量,显著提高了FG标记RGCs的密度,对TON后RGCs有保护作用。Objective To observe the protective effect of lycium barbarum polysaccharides(LBP)on retinal ganglion cells(RGCs)after optic nerve injury in adult rats.Methods 48 healthy male SD rats were randomly divided into four groups:normal group(A),injury group(B),LBP treatment group(C)and LBP prevention group(D),with 12 rats in each group.Except for group A,the other groups were used mini-clamp to make the injured optic nerve rat models on the left eye.Group D was given LBP 100 mg/(kg·d^(-1))for 2 weeks before modeling,and for 3 months after modeling.Group B and C were respectively given the normal saline and LBP 100 mg/(kg·d^(-1))for 3 months after modeling.Fluorescent gold(FG)was injected into the superior colliculi bilaterally to label RGCs one week before execution.The left eyeball of rats was removed after intragastric administration for three months.The retina was stripped and spread for calculating the density of RGCs.Meanwhile,the contents of superoxide dismutase(SOD)and malondialdehyde(MDA)in the retina were detected.Results The densities of RGC in group A,B,C and group D were(2354±250),(802±82),(1138±96)and(1196±82)cells/mm^(2) respectively.There was a statistically significant difference among the four groups(P<0.05).The density of RGCs in group B decreased,with different sizes and shapes,the staining of RGC was weak and their arrangement was irregular.The contents of SOD in group A,B,C and group D were(77.93±9.64),(24.23±7.44),(40.47±16.94)and(42.15±8.87)U/mgprot respectively.There was a statistically significant difference among the four groups(P<0.05).The contents of MDA in group A,B,C and group D were(6.21±1.97),(43.09±13.15),(29.55±4.51)and(28.91±6.74)nmol/mgprot respectively.There was a statistically significant difference among the four groups(P<0.05).Conclusion LBP enhances the ability to scavenge reactive oxygen species(ROS)by up-regulating the content of SOD in RGCs after TON,inhibits lipid peroxidation reaction and down-regulates the content of MDA.It significantly increases the density

关 键 词：外伤性视神经病变 视网膜神经节细胞 枸杞多糖 超氧化物歧化酶 丙二醛 

分 类 号：R774.6[医药卫生—眼科]