沉默肿瘤高表达细胞周期相关蛋白对肾癌细胞增殖和凋亡及β-catenin表达的影响  

Effects of silencing CREPT on the proliferation and apoptosis and β-catenin expression of renal cell carcinoma

作　　者：高裕[1] 赵霞[1] 王皓[1] 李广永[1] 李培军[1] 吕志勇[1] GAO Yu;ZHAO Xia;WANG Hao;LI Guangyong;LI Peijun;LYU Zhiyong(Department of Urology,General Hospital of Ningxia Medical University,Yinchuan 750004 China)

机构地区：[1]宁夏医科大学总医院泌尿外科,宁夏银川750004

出　　处：《宁夏医学杂志》2024年第11期949-953,F0003,共6页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(2019A0207)。

摘　　要：目的 探讨肿瘤高表达细胞周期相关蛋白(CREPT)在肾细胞癌及癌旁组织的表达情况及与常见临床病理因素的相关性,进一步研究沉默CREPT对肾细胞癌细胞增殖及凋亡的影响,同时探讨其对β-catenin表达的影响。方法 采用免疫组织化学法研究行根治性切除术的75对肾脏的透明细胞癌(RCCC)及癌旁组织芯片,探讨其在肾细胞癌、癌旁组织的表达情况并研究RCCC中CREPT和常见临床病理因素的相关性。同时应用基因沉默技术研究CREPT基因对肾细胞癌细胞的增殖及凋亡的影响,并探讨CREPT的沉默对786-O细胞中β-catenin表达的影响。结果 免疫组织化学显示CREPT在肾细胞癌高表达,在癌旁组织低表达,并且在病理分级临床分级分期具有差异性(P<0.05);沉默CREPT基因后抑制肾细胞癌细胞增殖,促进了肾癌细胞的凋亡(P<0.05);沉默CREPT基因后同时发现相较于对照组β-catenin表达未发现显著差异,p-β-catenin表达下调(P<0.05)。结论 沉默CREPT基因抑制了肾癌细胞的增殖,同时促进了肾癌细胞的凋亡;CREPT可能参与了Wnt/β-catenin信号通路促进肾肿瘤的生成。Objective To investigate the expression of CREPT in renal cell carcinoma and adjacent tissues and its correlation with common clinicopathological factors,to further study the effect of silencing CREPT on the proliferation and apoptosis of renal cell carcinoma cells,and to explore its relationship withβ-catenin relation.Methods 75 pairs of renal clear cell carcinoma and paracancerous tissue microarrays undergoing radical resection were collected and were examined by using immunohistochemistry technology,and its expression in renal cell carcinoma and paracancerous tissues were examined,and CREPT and common clinicopathological factors in renal cell carcinoma relevance was study.At the same time,gene silencing technology was used to study the effect of CREPT gene on the proliferation and apoptosis of renal cell carcinoma cells,and the effect of CREPT silencing on the expression ofβ-catenin in 786-O cells was explored.Results The results showed that CREPT was highly expressed in renal cell carcinoma and lower in adjacent tissues,and was different in pathological grading and clinical grading and staging(P<0.05);After silencing the CREPT gene,it was found that the proliferation of renal cell carcinoma was inhibited and promoted(P<0.05).After silencing the CREPT gene,it was found that the expression of p-β-catenin was down-regulated(P<0.05).Conclusion Silencing CREPT could inhibit the proliferation of renal cancer cells and promotes the apoptosis of renal cell cancer cells;CREPT may be involved in Wnt/β-catenin signaling pathway promotes renal tumorigenesis.

关 键 词：CREPT 肾细胞癌 免疫组织化学 细胞凋亡 Β-CATENIN 

分 类 号：R737.11[医药卫生—肿瘤]