孕期不同阶段补充维生素D对仔鼠学习记忆能力的影响  

Effects of vitamin D supplementation at different stages of pregnancy on learning and memory ability of offsprings

作　　者：孙晓霞 韩雨 乔瑜 侯雨霏 邱服斌 SUN Xiaoxia;HAN Yu;QIAO Yu;HOU Yufei;QIU Fubin(Department of Nutrition and Food Hygiene,School of Public Health,Shanxi Medical University,Taiyuan 030001,China;Institute of Nutrition and Food Science,Shanxi Medical University)

机构地区：[1]山西医科大学公共卫生学院营养与食品卫生学教研室,太原030001 [2]山西医科大学营养与食品科学研究所

出　　处：《山西医科大学学报》2024年第9期1191-1197,共7页Journal of Shanxi Medical University

基　　金：山西省回国留学人员科研资助项目(2022-114);山西省基础研究计划项目(202203021211227)。

摘　　要：目的 探索孕期不同阶段补充维生素D(vitamin D,VD)对后代仔鼠空间学习和记忆能力的改善作用。方法 按照体质量将4周龄SD雌性大鼠随机分为5组:对照组(control group,Ctrl组)、VD缺乏组(vitamin D deficiency group,VDD)、孕早期补充组(first trimester supplementation group,FTS)、孕中期补充组(second trimester supplementation group,STS)和孕晚期补充组(third trimester supplementation group,TTS),每组5只。Ctrl组采用标准饲料喂养,VDD组、FTS组、STS组和TTS组采用VD缺乏饲料喂养。喂养8周后,雌雄合笼,交配成功后,Ctrl组采用标准饲料喂养,VDD组采用VD缺乏饲料喂养,FTS组从妊娠0 d(GD0)开始采用标准饲料喂养,STS组从GD7开始时采用标准饲料喂养,TTS组从GD14开始时采用标准饲料喂养,直至仔鼠21 d离乳。所有仔鼠按照母鼠饲养条件喂养。GD20时检测母鼠血清25-羟基维生素D[25(OH)D]水平,仔鼠断乳后进行Morris水迷宫实验(包括定位航行实验4 d和空间探索实验1 d),于行为学实验结束后检测仔鼠血清25(OH)D、1,25-二羟基维生素D[1,25(OH)2D]水平,苏木精-伊红(HE)染色观察仔鼠海马体组织形态学变化。结果 VD缺乏8周后,与Ctrl组相比,VDD组、FTS组、STS组和TTS组母鼠血清25(OH)D水平均显著降低(P<0.01)。GD20时,与Ctrl组相比,VDD组母鼠血清25(OH)D水平显著降低(P<0.01);与VDD组相比,FTS组、STS组和TTS组母鼠血清25(OH)D水平均显著升高(P<0.01)。FTS组仔鼠血清25(OH)D水平显著高于VDD组(P<0.01),FTS组、STS组和TTS组仔鼠血清1,25(OH)2D水平显著高于VDD组(P<0.01)。孕期不同阶段补充VD后子代的体质量均明显增加。定位航行实验中,各组仔鼠随着训练天数的增加,逃避潜伏期逐渐降低;第3天时FTS组、STS组和TTS组仔鼠的逃避潜伏期均显著低于VDD组(P<0.01);与TTS组相比,FTS组和STS组逃避潜伏期都略有降低,但差异无统计学意义。空间探索实验中,FTS组和STS组仔鼠的逃避潜伏期显著�Objective To explore the improvement of vitamin D(VD)supplementation at different stages of pregnancy on spatial learning and memory ability in offspring mice.Methods According to body weight,female SD rats aged 4 weeks were randomized into control(Ctrl)group,vitamin D deficiency(VDD)group,first trimester supplementation(FTS)group,second trimester supplemen-tation(STS)group,and third trimester supplementation(TTS)group.The rats in Ctrl group were fed a standard diet,and the rats in the other groups were fed a VD deficiency diet.After 8 weeks of feeding,male and female rats were fed in the same cage.After successful mating,the rats in Ctrl group were fed with a standard diet,the rats in VDD group were fed with vitamin D deficient diet,the rats in FTS group were fed with standard diet from day 0 of gestation(GD0)to the ablactation(at day 21 of newborn rats),the rats in STS group were fed with a standard diet from GD7 to the ablactation,and the rats in TTS group were fed with a standard diet from GD14 to the ablactation.After the ablactation,all the offspring rats were fed according to the feeding conditions of maternal rats.At GD20,serum 25-hydroxyvitamin D[25(OH)D]level in maternal rats was detected by ELISA.Morris water maze test(four days of place navi-gation test and one day of spatial probe test)was carried out after weaning.Serum 25(OH)D and 1,25(OH)2D levels of the offsprings were detected after the behavioral experiment.Hippocampus morphology was observed by HE staining.Results After 8 weeks of VD deficiency,serum 25(OH)D level was significantly decreased in the other four groups compared with Ctrl group(P<0.01).At GD 20,serum 25(OH)D level was significantly lower in VDD group than in Ctrl group(P<0.01),and it was significantly higher in FTS group,STS group and TTS group than in VDD group(P<0.01).Serum 25(OH)D level in the offsprings in FTS group was significantly higher that in VDD group(P<0.01),and serum 1,25(OH)2D level in the offsprings in FTS group,STS group and TTS group was significantly higher than

关 键 词：维生素D 孕期维生素D缺乏 仔鼠 海马 MORRIS水迷宫 学习记忆能力 

分 类 号：R746.1[医药卫生—神经病学与精神病学]