胃癌组织中LncRNA PINK1-AS、miR-134-5p表达及与患者临床病理参数和预后的关系  

Expression of LncRNA PINK1-AS and miR-134-5p in gastric cancer tissues and their relationships with clinicopathological parameters and prognosis

作　　者：程灿 钟玉全[1] 杨丽[1] 张凤[1] CHENG Can;ZHONG Yuquan;YANG Li;ZHANG Feng(Department of Gastroenterology,The First People's Hospital of Neijiang,Neijiang 641000,China)

机构地区：[1]内江市第一人民医院消化内科,四川内江641000

出　　处：《山东医药》2024年第34期1-5,共5页Shandong Medical Journal

基　　金：四川省卫生健康科研课题立项项目(20PJ261)。

摘　　要：目的探讨胃癌组织中长链非编码核糖核酸(LncRNA)磷酸酯酶与张力蛋白同源物诱导激酶1反义核糖核酸(PINK1-AS)、微小核糖核酸-134-5p(miR-134-5p)表达及与患者临床病理参数和预后的关系。方法选取接受手术切除的胃癌患者104例,采用实时荧光定量聚合酶链式反应检测胃癌组织及对应癌旁组织中LncRNA PINK1-AS、miR-134-5p表达,分析胃癌组织中LncRNA PINK1-AS、miR-134-5p表达与临床病理参数的关系,采用Pearson法分析LncRNA PINK1-AS与miR-134-5p在胃癌组织中表达的相关性。根据胃癌组织LncRNA PINK1-AS、miR-134-5p表达的中位数分为LncRNA PINK1-AS、miR-134-5p高/低表达组,采用Kaplan-Meier法绘制生存曲线。通过Cox回归分析胃癌患者死亡的影响因素。结果胃癌组织中LncRNA PINK1-AS表达高于癌旁组织,miR-134-5p表达低于癌旁组织(P均<0.05)。LncRNA PINK1-AS与miR-134-5p在胃癌组织中表达呈负相关(r=-0.707,P<0.05)。不同组织分化程度、TNM分期、淋巴结转移的胃癌组织LncRNA PINK1-AS、miR-134-5p表达比较差异有统计学意义(P均<0.05)。随访3年,104例胃癌患者总生存率为61.54%(64/104)。LncRNA PINK1-AS高表达组3年总生存率低于LncRNA PINK1-AS低表达组,miR-134-5p高表达组3年总生存率高于miR-134-5p低表达组(P均<0.05)。胃癌患者死亡的独立危险因素为组织分化程度低分化、TNM分期Ⅲ期、有淋巴结转移、LncRNA PINK1-AS表达≥2.15,独立保护因素为miR-134-5p表达≥0.64(P均<0.05)。结论胃癌组织中LncRNA PINK1-AS高表达、miR-134-5p低表达,二者表达与组织分化程度降低、TNM分期增加、有淋巴结转移和预后不良有关。Objective To investigate the expression of long non-coding ribonucleic acid(LncRNA)phosphatase and tensin homolog induced kinase 1 antisense ribonucleic acid(PINK1-AS),and microribonucleic acid-134-5p(miR-134-5p)in the gastric cancer tissues,and their relationships with clinicopathological parameters and prognosis.Methods Totally 104 gastric cancer patients who underwent surgical resection were selected,and RT-qPCR was used to detect the expression of LncRNA PINK1-AS and miR-134-5p in the cancer tissues and corresponding adjacent tissues,and we analyzed the relationships between LncRNA PINK1-AS,miR-134-5p expression and clinicopathological parameters in gastric cancer tissues.The correlation between the expression of LncRNA PINK1-AS and miR-134-5p in gastric cancer tissues was analyzed by Pearson method.Gastric cancer tissues were divided into high/low LncRNA PINK1-AS and miR-134-5p expression groups according to their median LncRNA PINK1-AS and miR-134-5p expression,and various survival curves were plotted using the Kaplan-Meier method.The mortality factors of gastric cancer patients were analyzed by Cox regression.Results The expression of LncRNA PINK1-AS in the gastric cancer tissues was higher than that in the adjacent tissues,while the expression of miR-134-5p was lower than that in the adjacent tissues(both P<0.05).The expres sion of LncRNA PINK1-AS and miR-134-5p in gastric cancer tissues was negatively correlated(r=–0.707,P<0.05).There were statistically significant differences in the expression levels of LncRNA PINK1-AS and miR-134-5p in gastric cancer tissues with different degrees of differentiation,TNM staging,and lymph node metastasis(all P<0.05).At 3-year follow-up,the overall survival rate of 104 gastric cancer patients was 61.54%(64/104).The 3-year overall survival rate was lower in the high LncRNA PINK1-AS expression group than that in the low LncRNA PINK1-AS expression group,and the 3-year overall survival rate was higher in the high miR-134-5p expression group than that in the low miR-134-5p

关 键 词：胃癌 长链非编码核糖核酸磷酸酯酶与张力蛋白同源物诱导激酶1反义核糖核酸 微小核糖核酸-134-5p 临床病理参数 预后 

分 类 号：R735.2[医药卫生—肿瘤]