枯草芽胞杆菌ZWZ-19可分散油悬浮剂的研制及对马铃薯腐烂茎线虫病的防治效果  

Development and application of Bacillus subtilis ZWZ-19 dispersible oil suspension agent for Ditylenchus destructor

作　　者：车婷玉 李猛 赵远征 杨帆[1] 王振[1] 王东 周洪友[1] CHE Tingyu;LI Meng;ZHAO Yuanzheng;YANG Fan;WANG Zhen;WANG Dong;ZHOU Hongyou(Key Laboratory of Biopesticide Creation and Resource Utilization of Higher Education Institutions in Inner Mongolia Autonomous Region,Inner Mongolia Agricultural University,Hohhot 010018,China;Institute of Plant Protection,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古农业大学,生物农药创制与资源利用自治区高等学校重点实验室,呼和浩特010018 [2]内蒙古自治区农牧业科学院植物保护研究所,呼和浩特010031

出　　处：《植物保护》2024年第6期133-140,163,共9页Plant Protection

基　　金：国家自然科学基金(32360697);内蒙古自治区直属高校基本科研业务费(BR22-11-14);呼和浩特市高校院所协同创新项目(XTCX2023-19)。

摘　　要：为开发马铃薯腐烂茎线虫病的防治药剂,本研究利用前期获得的枯草芽胞杆菌ZWZ-19,制备一种可用于马铃薯拌种的可分散油悬浮剂。通过测定不同乳化剂对制剂稳定性的影响,结合制剂的悬浮率、倾倒性、粒径、静态表面张力及动态接触角等指标,筛选出ZWZ-19可分散油悬浮剂的最佳配方为:20%枯草芽胞杆菌母药、15%NP-10、3%有机土、0.5%乙二醇、3%白炭黑,大豆油补足至100%。该制剂热储前后悬浮率均高于95%,且有效成分分解率低于5%。田间试验结果表明,ZWZ-19可分散油悬浮剂对马铃薯腐烂茎线虫病的防效为69.61%,增产效果达到16.38%。To develop a control agent for Ditylenchus destructor,Bacillus subtilis ZWZ-19,obtained in our previous study was used to prepare a dispersible oil suspension formulation for seed-dressing of potatoes.By testing the effects of different emulsifiers on the stability of the formulation,combining with suspension rate,pourability,particle size,static surface tension,and dynamic contact angle,the optimal formula for the ZWZ-19 dispersible oil suspension was selected:20%Bacillus subtilis mother liquor,15%NP-10,3%OMMT,0.5%ethylene glycol,3%white carbon black,and soybean oil to make up 100%.The suspension rate of the formulation was above 95%before and after thermal storage,and the decomposition rate of the active ingredient was less than 5%.Field test results showed that the ZWZ-19 dispersible oil suspension had a control efficacy of 69.61%against stem rot nematode disease caused by Ditylenchus destructor,with a yield increase effect of 16.38%.

关 键 词：马铃薯 腐烂茎线虫 枯草芽胞杆菌 可分散油悬浮剂 防效 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]