超高效液相色谱-串联质谱法测定植物油中甲胺磷残留量的不确定度评定  

Evaluation of uncertainty in determination of methamidophos residue in vegetable oil by ultra-performance liquid chromatography-tandem mass spectrometry

作　　者：高含 刘锐 姜铖 王飞飞 GAO Han;LIU Rui;JIANG Cheng;WANG Feifei(Yunnan Yunke Characteristic Plant Extraction Laboratory Co.,Ltd.,Kunming,Yunnan 651000,China)

机构地区：[1]云南云科特色植物提取实验室有限公司,云南昆明651000

出　　处：《食品与机械》2024年第10期62-67,共6页Food and Machinery

基　　金：平台建设专项(云南实验室建设)(编号:2022YKZY001)。

摘　　要：[目的]保证农药甲胺磷残留检测质量。[方法]采用QuEChERS前处理方法、超高效液相色谱—串联质谱法检测一种植物油样品,测定样品的同时进行加标回收率测定,通过对试验过程中不确定度来源进行分析,对其测量不确定度进行评定。[结果]橄榄油样品中未检出甲胺磷残留量,对正确度1样品进行不确定度评定,结果为(0.018±0.010)mg/kg。[结论]在植物油中甲胺磷残留量的不确定度测量过程中,标准曲线拟合、超高液相色谱—质谱联用仪、标准工作溶液的影响相对较大,样品称量、样品前处理和样品重复性测定的影响相对较小。[Objective]To evaluation the uncertainty of determination of methamidophos residues in vegetable oil by ultra-performance liquid chromatography-tandem mass spectrometry.[Methods]A vegetable oil sample was detected by ultra-high performance liquid chromatography-tandem mass spectrometry,and the recovery rate was determined at the same time.By analyzing the sources of uncertainty in the experimental process,the measurement uncertainty was ultimately evaluated.[Results]The residue amount of methamidophos in olive oil samples was not detected,and the uncertainty of the accuracy 1 sample was evaluated,The results were(0.018±0.010)mg/kg.[Conclusion]In the uncertainty measurement process of methamidophos residue in vegetable oil,the influence of standard curve fitting,ultra-high liquid chromatography-mass spectrometry,and standard working solution is relatively large,while the influence of sample weighing,sample pretreatment,and sample repeatability determination is relatively small.

关 键 词：植物油 甲胺磷 液相色谱—串联质谱法 不确定度 

分 类 号：O657.63[理学—分析化学] TS227[理学—化学]