水蒸气蒸馏法提取八角中莽草酸及茴香油  

Extraction of shikimic acid and and anise oil from Illicium verum Hook.f.using steam distillation method

作　　者：王沈燕 李思美 李济甫 孟珂渏 杨洁[1,2,3] WANG Shenyan;LI Simei;LI Jifu;MENG Keyi;YANG Jie(College of Nursing and Health Management&College of Life Science and Chemistry,Wuhan Donghu University,Wuhan,Hubei 430212,China;Innovation Institute for Biomedical Materials,Wuhan Donghu University,Wuhan,Hubei 430212,China;College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan,Hubei 430074,China)

机构地区：[1]武汉东湖学院护理与健康管理学院(生命科学与化学学院),湖北武汉430212 [2]武汉东湖学院生物医用材料创新研究所,湖北武汉430212 [3]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《食品与机械》2024年第10期174-180,共7页Food and Machinery

基　　金：湖北省自然科学基金项目(编号:2022CFB197);湖北省高校优秀中青年科技创新团队项目(编号:T2022048);2022年度“东湖未来之星”大学生科研基金重点项目(编号:4)。

摘　　要：[目的]提高八角产业的附加值。[方法]通过水蒸气蒸馏分离出八角中的茴香油,去油后的粗提液经离子交换法纯化得高纯度的莽草酸。在蒸馏过程中确定了八角粉粒度、料液比、水蒸气蒸馏次数等参数,在莽草酸分离纯化过程中,采用单因素试验和响应面试验优选了上样溶液pH、洗脱流量、洗脱液HCl体积分数,并将水蒸气蒸馏法与热回流法进行了对比。[结果]八角粉粒度为100目、料液比1∶15(g/mL),水蒸气蒸馏2次后茴香油的产率高达13.55%;离子交换纯化过程中,上样溶液pH为4.0、洗脱流量为27 mL/h、洗脱液HCl体积分数为4.40%时莽草酸产率达11.05%,纯度在98.50%以上。[结论]该方法实现了高产率综合提取两种活性成分的目标。[Objective]This study aimed to improve the added value of the industry of Illicium verum Hook.f.[Methods]The anise oil in I.verum was separated by steam distillation,and the high-purity shikimic acid was obtained from the crude by ion exchange.During the distillation process,parameters such as particle size of I.verum powder,material liquid ratio,and number of steam distillation times were determined.In the separation and purification process of shikimic acid,single factor experiments and response surface methodology were used to optimize the pH of the sample solution,elution flow rate,and volume fraction of HCl of the elution solution.The steam distillation method was compared with the thermal reflux method.[Results]The yield of anise oil reached 13.55%with the particle size of I.verum powder(100 mesh),the material liquid ratio 1∶15(g/mL),and steam distillation twice;In the ion exchange purification process,when the pH of the sample solution was 4.0,the elution flow rate was 27 mL/h,and the volume fraction of HCl of the eluent was 4.40%,the yield and purity of shikimic acid reached 11.05%and 98.50%,respectively;The steam distillation method had more industrial value.[Conclusion]This method achieved the goal of high-yield rate comprehensive extraction of two active ingredients.

关 键 词：八角 茴香油 莽草酸 水蒸气蒸馏法 分离纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学] TS225.3[轻工技术与工程—食品科学与工程]