HBB通过调控Wnt/β-catenin信号通路抑制甲状腺未分化癌发展  

HBB inhibits the development of anaplastic thyroid cancer by regulating Wnt/β-catenin signaling pathway

作　　者：郭宏鹏 张凤坤[2] 李尤 张金辉 潘星合 张睿 张艺彤 刘俊良 孙成林[2] GUO Hongpeng;ZHANG Fengkun;LI You;ZHANG Jinhui;PAN Xinghe;ZHANG Rui;ZHANG Yitong;LIU Junliang;SUN Chenglin(Department of General Surgery,The Second Hosipital Affiliated to Shenyang Medical College,Shenyang 110002,China;Department of General Surgery,The Central Hosipital Affiliated to Shenyang Medical College)

机构地区：[1]沈阳医学院附属第二医院普外科,辽宁沈阳110002 [2]沈阳医学院附属中心医院普外一科

出　　处：《沈阳医学院学报》2024年第6期570-574,596,共6页Journal of Shenyang Medical College

基　　金：辽宁省科学技术计划项目(No.2022JH2/101300035)。

摘　　要：目的:探究HBB在甲状腺未分化癌(anaplastic thyroid cancer,ATC)细胞中表达情况及其对ATC细胞增殖、侵袭、迁移和凋亡的调控作用和潜在机制。方法:通过TIMER数据库分析HBB在甲状腺癌及癌旁组织中表达情况;通过KM-plotter数据库分析HBB表达与甲状腺癌患者总生存期相关性;通过RT-qPCR检测HBB mRNA在ATC细胞中表达情况;将HBB敲减或过表达质粒转染甲状腺未分化癌细胞,通过Western blot检测细胞HBB蛋白表达,通过CCK-8检测细胞增殖活力,通过Transwell检测细胞迁移和侵袭能力,通过流式细胞术检测细胞凋亡,通过Western blot检测细胞中β-catenin表达。结果:HBB在甲状腺癌组织中低表达,且其高表达患者总生存期高。HBB蛋白在ATC细胞中表达下调。敲减HBB增加甲状腺未分化癌细胞增殖、迁移和侵袭能力及β-catenin蛋白表达水平,抑制凋亡;而过表达HBB降低甲状腺未分化癌细胞增殖、迁移和侵袭能力及β-catenin蛋白表达水平,促进凋亡。结论:HBB高表达与甲状腺癌患者较高的总生存期相关。其可能通过Wnt/β-catenin信号通路抑制ATC细胞增殖、迁移和侵袭,促进凋亡。Objective:To investigate the expression of HBB in anaplastic thyroid cancer(ATC)cells and its regulatory effect on proliferation,invasion,migration and apoptosis of ATC cells and its potential mechanism.Methods:The expression of HBB in thyroid cancer and paracancerous tissues was analyzed through TIMER database.The correlation between the expression of HBB and the overall survival time of thyroid cancer patients was analyzed through KM-plotter database.The expression of HBB mRNA in ATC cells was detected by RT-qPCR.The HBB knockout or overexpression plasmid was transfected into ATC cells.The expression of HBB protein was detected by Western blot.The proliferation activity was detected by CCK-8 assay.The migration and invasion ability was detected by Transwell assay.The apoptosis was detected by flow cytometry.The expression ofβ-catenin was detected by Western blot.Results:The expression of HBB was low in thyroid cancer,and the overall survival time of patients with high expression of HBB was high.The expression of HBB protein was down-regulated in ATC cells.Knockout of HBB increased the ability of proliferation,migration and invasion of ATC cells and the expression ofβ-catenin protein,and inhibited apoptosis.However,overexpression of HBB decreased the ability of proliferation,migration and invasion of ATC cells and the expression ofβ-catenin protein,and promoted apoptosis.Conclusions:High HBB expression is associated with higher overall survival in patients with thyroid cancer.It may inhibit the proliferation,migration and invasion of ATC cells and promote apoptosis through Wnt/β-catenin signal pathway.

关 键 词：甲状腺未分化癌 HBB 增殖 迁移和侵袭 凋亡 WNT/Β-CATENIN信号通路 

分 类 号：R736.1[医药卫生—肿瘤]