火炭母提取物激活NF-κB抑制AMPK通路诱导人卵巢癌SK-OV-3细胞凋亡研究  

Study on the apoptosis induction of human ovarian cancer SK-OV-3 cells by activating NF-kB and inhibiting AMPK pathway with extracts from polygonum chinense

作　　者：罗德平 肖威 李慧娟 吕根梅 余瑛 LUO Deping;XIAO Wei;LI Huijuan;LYU Genmei;YU Ying(Ganzhou Cancer Hospital,Ganzhou,Jiangri 3410o0,China)

机构地区：[1]赣州市肿瘤医院妇瘤科,江西赣州341000 [2]赣州市肿瘤医院中医科,江西赣州341000 [3]赣州市肿瘤医院病理中心,江西赣州341000 [4]赣州市肿瘤医院临床药学中心,江西赣州341000

出　　处：《社区医学杂志》2024年第16期551-556,共6页Journal Of Community Medicine

基　　金：江西省中医院药管理局科技计划课题(2021B016)。

摘　　要：目的 研究火炭母提取物对人卵巢癌SK-OV-3细胞凋亡的影响及其可能的分子机制。方法 利用乙酸乙酯萃取火炭母有效成分,用MTT法检测火炭母提取物对人卵巢癌SK-OV-3细胞增殖的影响,流式细胞仪检测细胞周期、细胞凋亡,利用蛋白质印迹法检测核转录因子κB(NF-κB)及腺苷酸活化蛋白激酶(AMPK)通路相关蛋白。结果 火炭母乙酸乙酯提取物能够抑制人卵巢癌SK-OV-3细胞增殖能力,且抑制率与处理浓度和时间增加而增强,火炭母浓度为10 mg/L时,24、48和72 h卵巢癌细胞抑制增殖率的平均值为3.37%、4.21%及5.51%;火炭母浓度为100 mg/L时,24、48和72 h卵巢癌细胞抑制增殖率的平均值为38.86%、45.86%及54.54%;火炭母浓度为1 000 mg/L时,24、48和72 h卵巢癌细胞抑制增殖率的平均值为65.39%、73.76%及84.97%,差异有统计学意义,均P<0.01。0、10、20、50、100、1 000 mg/L的火炭母乙酸乙酯提取物作用于人卵巢癌SK-OV-3细胞后24 h使用流式细胞术检测细胞凋亡率P值分别为0.977、0.013、0.008、0.003和0.012,显示20 mg/L以上的火炭母乙酸乙酯提取物处理导致SK-OV-3细胞凋亡率显著升高。蛋白质印记法实验结果表明,随火炭母乙酸乙酯提取物处理浓度增加,SK-OV-3细胞NF-κB p65蛋白表达水平升高,而AMPK蛋白表达水平下降。结论 火炭母乙酸乙酯提取物可能通过激活NF-κB通路,抑制AMPK蛋白的表达,诱导人卵巢癌SK-OV-3细胞凋亡。火炭母乙酸乙酯提取物对人卵巢癌SK-OV-3细胞的毒性作用呈明显的剂量依赖性和时间依赖性。Objective To investigate the effect of polygonum chinense extract on apoptosis of human ovarian cancer SK-OV-3 cells and its possible molecular mechanism.Methods The active ingredients were extracted with ethyl acetate,and the effects of the extract on the proliferation of human ovarian cancer SK-OV-3 cells were detected by MTT,the cell cycle and apoptosis were detected by flow cytometry,and the Nuclear factor kappa B(NF-kB)and AMP-activated protein kinase(AMPK)pathway-related proteins were detected by protein blotting.ResultssEthyl acetate extract of polygonum chinense could inhibit the proliferation of human ovarian cancer SK-OV-3 cells,and the inhibition rate increased with treatment concentration and time.When the concentration of Polygonum chinense was 10 mg/L,the mean inhibition rates of ovarian cancer cell proliferation at 24,48 and 72 hours were 3.37%,4.21%and 5.51%;when the concentration was 100 mg/L,the average inhibition rates of ovarian cancer cell proliferation at 24,48 and 72 hours were 38.86%,45.86%and 54.54%;when the concentration was 1000 mg/L,the average inhibition rates at 24,48 and 72 hours were 65.39%,73.76%and 84.97%,with all P<0.01.The P values for apoptosis rates detected by flow cytometry 24 h after treatment with 0,10,20,50,100,and 1,000 mg/L of polygonum chinense ethyl acetate extract were 0.977,0.013,0.008,0.003,and 0.012,respectively,indicating that the apoptosis rate of SK-OV-3 cells significantly increased when treated with more than 20 mg/L of polygonum chinense ethyl acetate extract.Western blot results showed that the expression level of NF-kB p65 protein in SK-OV-3 cells increased with the increase of ethyl acetate extract concentration,while the expression level of AMPK protein decreased.Conclusions The ethyl acetate extract of polygonum chinense may induce apoptosis in human ovarian cancer SK-OV-3 cells by activating the NF-sB pathway and inhibiting the expression of AMPK protein.The toxic effects of ethyl acetate extract of Polygonum chinense on human ovarian cancer SK-OV-3 c

关 键 词：火炭母 核转录因子ΚB 腺苷酸活化蛋白激酶 细胞凋亡 人卵巢癌SK-OV-3细胞 

分 类 号：R737.31[医药卫生—肿瘤]