老年食管癌患者放疗疗效与GPR37甲基化状态关系的临床研究  

Clinical study on the relationship between the efficacy of radiotherapy and the methy -lation status of GPR37 in elderly patients with esophageal cancer

作　　者：孟芳 强金虎 顾科 杨璐 高萌 张世强 MENG Fang;QIANG Jinhu;GU Ke;YANG Lu;GAO Meng;ZHANG Shiqiang(Department of Oncology and Haematology,Wuxi Branch of Zhongda Hospital Affiliated to Southeast University&Xishan People′s Hospital,Wuxi 214105;Department of Oncology and Radiotherapy,Affiliated Hospital of Jiangnan University,China)

机构地区：[1]东南大学附属中大医院无锡分院&无锡市锡山人民医院肿瘤血液科,江苏无锡214105 [2]江南大学附属医院肿瘤放射治疗科

出　　处：《胃肠病学和肝病学杂志》2024年第12期1631-1635,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：无锡市卫生健康委科研项目计划(ZH202106);无锡市“太湖人才计划”医疗卫生高层次人才项目;无锡市锡山人民医院院级科研项目创新培育基金课题(医202105)。

摘　　要：目的探讨老年食管癌患者放疗疗效与G蛋白偶联受体37(G protein-coupled receptor 37,GPR37)甲基化状态之间的关系。方法选择2023年1月至2023年6月我院收治的66例老年食管癌患者为研究对象,取其癌组织和相应的癌旁组织样本。采用免疫组织化学法测定GPR37的表达,特异性PCR法检测GPR37基因甲基化。分析老年食管癌患者放疗疗效与GPR37甲基化状态的关系。结果与癌旁组织相比,癌组织GPR37阳性及其基因甲基化阳性率明显较高(P<0.05);GPR37阳性及其基因甲基化阳性均与老年食管癌患者的分化程度、TNM分期、淋巴结转移密切相关(P<0.05);单因素和多因素Logistic回归分析结果显示,分化程度、TNM分期、淋巴结转移、GPR37基因甲基化阳性均会影响老年食管癌患者放疗疗效(P<0.05)。结论GPR37阳性及其基因甲基化阳性率在老年食管癌患者癌组织中明显较高,且与分化程度、TNM分期、淋巴结转移有关;此外,老年食管癌患者放疗疗效也与分化程度、TNM分期、淋巴结转移、GPR37基因甲基化阳性有一定的关系。Objective To explore the relationship between the efficacy of radiotherapy and the methylation status of G protein-coupled receptor 37(GPR37)in elderly patients with esophageal cancer.Methods Sixty-six elderly patients with esophageal cancer admitted to our hospital from Jan.2023 to Jun.2023 were selected as the research subjects,and their cancer tissue and corresponding adjacent tissue samples were collected.Immunohistochemistry was used to determine the expression of GPR37,and specific PCR was used to detect GPR37 gene methylation.To analyze the relationship between the efficacy of radiotherapy and GPR37 methylation status in elderly patients with esophageal cancer.Results Compared with adjacent tissues,the positive rate of GPR37 positivity and gene methylation in cancer tissue was significantly higher(P<0.05);both GPR37 positivity and gene methylation positivity were related to the differentiation degree and TNM stage of elderly esophageal cancer patients,lymph node metastasis were closely related(P<0.05);the results of single-factor and multi-factor Logistic regression analysis showed that the degree of differentiation,TNM stage,lymph node metastasis and GPR37 gene methylation positivity would affect the efficacy of radiotherapy in elderly patients with esophageal cancer(P<0.05).Conclusion GPR37 positivity and its gene methylation positivity rate are significantly higher in cancer tissues of elderly patients with esophageal cancer,and are related to the degree of differentiation,TNM stage and lymph node metastasis.In addition,the efficacy of radiotherapy in elderly patients with esophageal cancer is also related to the degree of differentiation,TNM stage and lymph node metastasis.There is a certain relationship between metastasis and GPR37 gene methylation positivity.

关 键 词：食管癌 放疗疗效 G蛋白偶联受体37 甲基化 临床特征 

分 类 号：R735.1[医药卫生—肿瘤]