甲状腺激素T3对急性酒精性肝损伤小鼠肝脏细胞增殖与凋亡的影响  

Effects of thyroid hormone T3 on proliferation and apoptosis of liver cells in mice with acute alcoholic liver injury

作　　者：孙枫林 罗仁利 冯家阳 李昊远 吴俊菲 李三强[1] SUN Fenglin;LUO Renli;FENG Jiayang;LI Haoyuan;WU Junfei;LI Sanqiang(Key Laboratory of Molecular Medicine for Liver Injury and Repair,College of Basic Medicine and Law,He′nan University of Science and Technology,Luoyang 471000;Department of Emergency,He′nan University of Science and Technology Affiliated Xuchang Central Hospital,China)

机构地区：[1]河南科技大学基础医学与法医学院肝脏损伤与修复分子医学重点实验室,河南洛阳471000 [2]河南科技大学附属许昌市中心医院急诊科

出　　处：《胃肠病学和肝病学杂志》2024年第12期1661-1664,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然基金资助项目(82170606);河南省高等学校重点科研项目计划基础研究专项(23ZX006)。

摘　　要：目的研究甲状腺激素T3对急性酒精性肝损伤小鼠的肝脏结构与功能的影响及细胞增殖与凋亡的相关基因表达。方法对正常小鼠提前给予不同浓度的甲状腺激素T3腹腔注射干预,最后一次给药后给予50%酒精溶液一次性灌胃,24 h后处死,并留取血清和肝脏。该实验通过建立小鼠急性酒精性肝损伤模型,对甲状腺激素T3在其中的作用进行分析。结果经T3干预后的急性酒精性肝损伤小鼠可观察到肝小叶轮廓清晰,肝索排列较为整齐,但存在炎细胞浸润,血清ALT活性和肝脏ROS含量增加,高剂量组PCNA表达增加(P<0.05),凋亡基因Caspase3和Bax表达较模型组有所降低(P<0.01),但随T3干预浓度升高凋亡基因表达出现上升趋势。结论浓度较高的甲状腺激素T3干预,会使小鼠急性酒精性肝损伤加重。Objective To study the effects of thyroid hormone T3 on the liver structure and function,as well as the expression of genes related to cell proliferation and apoptosis in mice with acute alcoholic liver injury.Methods Normal mice were intervened by intraperitoneal injection of different concentrations of thyroid hormone T3 in advance.After the last dose,50%alcohol solution was administered once by gavage.Twenty-four hours post-treatment,the mice were euthanized,and both serum and liver samples were collected.This experiment sought to establish a mouse model of acute alcoholic liver injury and assess the influence of thyroid hormone T3 on this condition.Results After T3 intervention,more severe damage in mice with acute alcoholic liver injury could be observed,clear liver lobule contours and neat arrangement of liver cords.But there was infiltration of inflammatory cells,increased serum ALT activity and liver ROS content,increased PCNA expression in the high-dose group(P<0.05),and decreased expression of apoptotic genes Caspase3 and Bax compared to model group(P<0.01).However,with the increase of T3 intervention concentration,the expression of apoptotic genes showed an upward trend.Conclusion Intervention with high concentrations of thyroid hormone T3 could exacerbate acute alcoholic liver injury in mice.

关 键 词：甲状腺激素T3 急性酒精性肝损伤 细胞增殖与凋亡 

分 类 号：R575[医药卫生—消化系统]