基于大肠杆菌表达系统高效生产外源蛋白的自诱导培养基的研发  

Development of self-induction medium for efficient production of recombinant protein based on Escherichia coli expression system

作　　者：侯茁 邵军军[1] 刘伟 高闪电[1] 彭德财 宁小雨 马云云 温升辉 常惠芸[1] HOU Zhuo;SHAO Junjun;LIU Wei;GAO Shandian;PENG Decai;NING Xiaoyu;MA Yunyun;WEN Shenghui;CHANG Huiyun(Gansu Provincial Research Center for Basic Discipline of Pathogenic Biology/OIE/National Reference Laboratory for Foot-and-Mouth Disease/State Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]中国农业科学院兰州兽医研究所动物疫病防控国家重点实验室OIE/国家口蹄疫参考实验室甘肃省病原生物学基础学科研究中心,甘肃兰州730046

出　　处：《中国兽医科学》2024年第11期1430-1436,共7页Chinese Veterinary Science

基　　金：兰州市科技项目(2021-1-12);甘肃省青年科技基金项目(21JR7RA025)。

摘　　要：为提高大肠杆菌生产抗原的产量,实现企业降本增效,加快口蹄疫多表位疫苗的产业化进程,本研究拟研发适合大肠杆菌表达系统高效表达重组蛋白的自诱导培养基,并以实验室构建的口蹄疫多表位重组表达质粒为试验对象,通过正交试验、优化诱导温度,筛选和确定最佳自诱导培养基配伍。结果显示,在16℃诱导培养,自诱导培养基表达可溶性蛋白的产量为普通LB培养基的6倍,而成本却降低了78%。这说明我们成功研发出了适合原核表达系统的自诱导培养基,其良好的成本效益性价比将有助于生物制品生产企业降低成本,提高产品的竞争力,具有广阔应用前景的产品。The self-induction medium suitable for the expression system of Escherichia coli to efficiently express recombinant protein was developed to increase the production of antigens,reduce costs and increase efficiency for enterprises.This study will be developed self-induction medium suitable for Escherichia coli expression system to express recombinant protein efficiently.The recombinant expression plasmid with the gene of multi-epitope for foot-and-mouth disease antigen developed previously in our laboratory was used to verification of self-inducing medium.An optimal self-inducing culture medium was developed through orthogonal experiment and suitable induction temperature.The results showed that the yield of foot-and-mouth disease antigen produced by highly efficient self-induced medium was 6 times that of ordinary LB medium,and the cost of producing 1 mg foot-and-mouth disease antigen was reduced by 78%.This indicated that we have successfully developed a self-induction medium suitable for prokaryotic expression systems.The good cost-effectiveness will help biological products enterprises reduce costs,improve product competitiveness,and create products with broad application prospects.

关 键 词：大肠杆菌 表达 自诱导培养基 乳糖 

分 类 号：S855.3[农业科学—临床兽医学]