基于SSR分子标记的盐肤木遗传多样性分析  

Genetic diversity analysis of Rhus chinensis based on SSR markers

作　　者：蔡彦君 吴雯雯 杨冰冰 张霞 刘文 何正权 桑子阳 CAI Yanjun;WU Wenwen;YANG Bingbing;ZHANG Xia;LIU Wen;HE Zhengquan;SANG Ziyang(Key Laboratory of Plant Genetics and Germplasm Innovation in the Three Gorges Region,Biotechnology Research Center of Three Gorges University,Yichang 443002,Hubei,China;Forestry Bureau of Wufeng Tujia Autonomous County,Wufeng Tujia Autonomous County 443400,Hubei,China)

机构地区：[1]三峡大学生物技术研究中心三峡区域植物遗传与种质创新重点实验室,湖北宜昌443002 [2]五峰土家族自治县林业局,湖北五峰土家族自治县443400

出　　处：《生物资源》2024年第5期478-486,共9页Biotic Resources

基　　金：湖北省中央引导地方发科技发展专项(2022BGE265);国家自然科学基金面上项目(32370393)。

摘　　要：利用筛选出的14对SSR引物,对4省5个地区的153份盐肤木种质资源进行遗传多样性分析,并进行遗传关系及聚类分析。结果显示,14对SSR引物在153份样本中扩增出105个等位位点,平均每对引物扩增到的等位位点数为7.5个。盐肤木Nei’s多样性指数(H)为0.206~0.413,平均值为0.316;Shannon多样性信息指数(I)为0.356~0.600,平均值为0.484。5个居群间的有效等位基因数(Ne)为1.335~1.556,Nei’s多样性指数(H)0.197~0.316,Shannon多样性信息指数(I)为0.302~0.467,居群的遗传分化系数(Gst)变化范围0.122~0.489,平均值为0.257,基因流的变化范围(Nm)为0.522~3.594,平均值为1.445(Nm>1)。结果表明,盐肤木在物种水平上遗传多样性丰富,居群间遗传多样性存在一定差异,25.7%的总体变异发生在居群间,74.3%的遗传变异发生在居群内,即盐肤木的遗传分化主要存在于居群内部,居群间存在着较频繁的基因交流。遗传多样性系数及聚类分析结果显示,5个居群被聚为两类,盐肤木优先以居群聚类,盐肤木的遗传关系与地理位置之间有一定的相关性。该研究结果可以对盐肤木后期优良种质的选育提供参考依据。In order to study the genetic diversity of Rhus chinensis Mill.population and lay a theoretical foundation for the rational management,protection and utilization of its germplasm resources,the genetic diversity of 153 germplasm resources from 5 regions in 4 provinces was studied using 14 pairs of SSR primers.Genetic diversity parameters were cal-culated,and the genetic relationship and cluster analysis were carried out.14 pairs of SSR primers amplified 105 alleles in 153 samples,with an average of 7.5 alleles amplified by each primer pair.Nei’s diversity index(H)ranged from 0.206 to 0.413,with an average value of 0.316.The Shannon diversity information index(I)ranged from 0.356 to 0.600,with an average value of 0.484.The effective alleles(Ne),Nei's diversity index(H)and Shannon diversity information index(I)were 1.335-1.556,0.197-0.316 and 0.302-0.467,respectively.The genetic differentiation coefficient(Gst)of the population was 0.257,and the inter-population gene flow was 1.445(Nm>1).The coefficient of genetic diversity and cluster analysis showed that the 5 populations were clustered into two categories,with Rhus chinensis priori-tizing clustering based on population.The results show that the genetic diversity of Rhus chinensis is high,the genetic variation mainly exists within the population,and there are frequent gene exchanges among the populations.The genetic relationship of Rhus Chinensis is related to the geographical location.This study can provide reference for the breeding of excellent germplasm of Rhus chinensis in later period.

关 键 词：盐肤木 简单重复序列 遗传多样性 聚类分析 

分 类 号：Q341[生物学—遗传学]