线粒体代谢紊乱和免疫浸润在癫痫中的作用  

Bio the role of mitochondrial metabolic disorder and immune infiltration in epilepsy

作　　者：马跃 龚星源 刘浩 董金玉 胡忠波[1] 刘永良[1] Ma Yue;Gong Xingyuan;Liu Hao;Dong Jinyu;Hu Zhongbo;Liu Yongliang(Department of Neurosurgery,Binzhou Medical University Hospital,Shandong 256603,China)

机构地区：[1]滨州医学院附属医院神经外科,山东256603 [2]成都医学院第二附属医院重症医学科

出　　处：《脑与神经疾病杂志》2024年第12期774-780,共7页Journal of Brain and Nervous Diseases

基　　金：山东省自然科学基金青年基金(ZR2020QH104)。

摘　　要：目的基于生物信息学方法,探讨癫痫发作中线粒体代谢与免疫微环境的相互作用机制。方法研究者从GEO数据库获取癫痫相关数据集,结合MitoCarta3.0数据库筛选出差异表达基因(DEGs),特别是与线粒体功能相关的MitoDEGs。随后进行通路分析,构建PPI网络,确定关键中枢MitoDEG,并预测其转录因子和靶miRNA。此外,利用ImmuCellAI分析免疫微环境,探讨MitoDEGs与免疫细胞浸润的关系。结果研究发现,在癫痫中,MitoDEGs显著富集于线粒体代谢和免疫调节通路。识别出5个关键中枢MitoDEG,并发现癫痫患者免疫微环境中巨噬细胞浸润增加。特定MitoDEGs与免疫细胞存在相关性。结论该研究成功揭示了癫痫发作中线粒体代谢与免疫微环境的相互作用,为深入理解癫痫发病机理及寻找新治疗策略提供了重要线索。Objective This study aims to explore the interaction mechanism between mitochondrial metabolism and immune microenvironment in epileptic seizures based on bioinformatics methods.Methods Researchers obtained epilepsy-related datasets from the GEO database,and screened differentially expressed genes(DEGs),especially MitoDEGs related to mitochondrial function,in combination with the MitoCarta3.0 database.Subsequently,they conducted pathway analysis,constructed PPI networks,identified key hub MitoDEGs,and predicted their transcription factors and target miRNAs.Additionally,they utilized ImmuCellAI to analyze the immune microenvironment and investigate the relationship between MitoDEGs and immune cell infiltration.Results The study found that MitoDEGs were significantly enriched in mitochondrial metabolism and immune regulation pathways in epilepsy.Five key hub MitoDEGs were identified,and an increase in macrophage infiltration was observed in the immune microenvironment of epileptic patients.Specific MitoDEGs were found to correlate with immune cells.Conclusion This study successfully revealed the interaction between mitochondrial metabolism and immune microenvironment in epileptic seizures,providing important clues for a deeper understanding of the pathogenesis of epilepsy and the search for new treatment strategies.

关 键 词：生物信息学分析 癫痫 免疫浸润 免疫代谢 线粒体 

分 类 号：R742.1[医药卫生—神经病学与精神病学]