Genomic and metabolomic insights into the selection and differentiation of bioactive compounds in citrus  

作　　者：Xiao Liang Yue Wang Wanxia Shen Bin Liao Xiaojuan Liu Zimeng Yang Jiebiao Chen Chenning Zhao Zhenkun Liao Jinping Cao Ping Wang Peng Wang Fuzhi Ke Jianguo Xu Qiong Lin Wanpeng Xi Lishu Wang Juan Xu Xiaochun Zhao Chongde Sun 

机构地区：[1]Plant Growth,Development and Quality Improvement,Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants,Zhejiang University,Hangzhou,China [2]Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing,China [3]Hainan Institute of Zhejiang University,Sanya,China [4]Zhejiang Citrus Research Institute,Taizhou,China [5]Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process,Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing,China [6]College of Horticulture and Landscape Architecture,Southwest University,Chongqing,China [7]Department of Hematology and Hematopoietic Cell Transplantation,Comprehensive Cancer Center,City of Hope National Medical Center,Duarte,CA,USA [8]Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan,China

出　　处：《Molecular Plant》2024年第11期1753-1772,共20页分子植物（英文版）

基　　金：supported by the National Key Research and Development Program of China(2023YFD2300604);the National Natural Science Foundation of China(32072132,32101932);the Hangzhou Joint Fund of the Zhejiang Provincial Natural Science Foundation of China(LHZSD24C150001);the Fundamental Research Funds for the Zhejiang Provincial Universities(226-2024-00211);the Fundamental Research Funds for the Central Universities(226-2024-00235).

摘　　要：Bioactive compounds play an increasingly prominent role in breeding functional and nutritive fruit crops such as citrus.However,the genomic and metabolic bases for the selection and differentiation underlying bioactive compound variations in citrus remain poorly understood.In this study,we constructed a species-level variation atlas of genomes and metabolomes using 299 citrus accessions.A total of 19829 significant SNPs were targeted to 653 annotated metabolites,among which multiple significant signals were identified for secondary metabolites,especially flavonoids.Significant differential accumulation of bioactive compounds in the phenylpropane pathway,mainly flavonoids and coumarins,was unveiled across ancestral citrus species during differentiation,which is likely associated with the divergent haplotype distribution and/or expression profiles of relevant genes,including p-coumaroyl coenzyme A 2′-hydroxylases,flavone synthases,cytochrome P450 enzymes,prenyltransferases,and uridine diphosphate glycosyltransferases.Moreover,we systematically evaluated the beneficial bioactivities such as the antioxidant and anticancer capacities of 219 citrus varieties,and identified robust associations between distinct bioactivities and specific metabolites.Collectively,these findings provide citrus breeding options for enrichment of beneficial flavonoids and avoidance of potential risk of coumarins.Our study will accelerate the application of genomic and metabolic engineering strategies in developing modern healthy citrus cultivars.

关 键 词：CITRUS METABOLOME genome-wide association study bioactive compounds 

分 类 号：Q94[生物学—植物学]