克氏原螯虾促雄性腺摘除前后血淋巴外泌体差异蛋白组学分析  

Differential Proteomic Analysis of Exosomes in Hemolymph of Procambarus clarkii Before and After Androgenic Gland Ablation

作　　者：杨思琦 王齐帅 李艳和 YANG Siqi;WANG Qishuai;LI Yanhe(Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education/Hubei Hongshan laboratory/College of Fisheries,Huazhong Agricultural University,Wuhan 430070,Hubei,China)

机构地区：[1]华中农业大学水产学院/长江经济带大宗水生生物产业绿色发展教育部工程研究中心/湖北洪山实验室,武汉430070

出　　处：《农学学报》2024年第11期56-63,共8页Journal of Agriculture

基　　金：中央高校基本科研业务费专项资金资助项目(2662020SCPY004);湖北省重点研发计划项目“小龙虾优良品系(种)培育及其关键技术研究”(2021BBA232)。

摘　　要：为了了解可能参与克氏原螯虾性腺分化的相关调控因子,对克氏原螯虾促雄性腺摘除前后血淋巴外泌体进行了蛋白组测序和分析。结果表明,在摘除促雄性腺前后血淋巴外泌体中一共鉴定到蛋白质469个,差异表达蛋白98个。14-3-3 zeta和cell division cycle protein 27 homolog这2个差异表达蛋白可能参与了克氏原螯虾的性别调控。In order to understand the regulation factors that may be involved in gonadal differentiation of P.Procambarus,in this study,the proteome of exosomes in the hemolymph before and after androgenic gland ablation of P.clarkii was sequenced and analyzed.The results showed that a total of 469 proteins were identified in hemolymph exosomes,and 98 differentially expressed proteins were screened.Among which two differentially expressed proteins,14-3-3 zeta and cell division cycle protein 27 homolog,may be involved in the sex regulation of P.clarkii.

关 键 词：克氏原螯虾 促雄性腺 外泌体 蛋白组 

分 类 号：S966.12[农业科学—水产养殖]