circFNDC3B调控巨噬细胞极化对口腔鳞状细胞癌细胞凋亡和GPNMB/CD44轴的作用机制  

Mechanism of circFNDC3B regulation of macrophage polarization on apoptosis and GPNMB/CD44 axis in oral squamous cell carcinoma cells

作　　者：易笑冉 张乾 石佳佩 严梦 刘俊杰 YI Xiao-ran;ZHANG Qian;SHI Jia-pei;YAN Meng;LIU Jun-jie(Huazhong University of Science and Technology Tongji Medical College Affiliated Union Hospital,Wuhan,Hubei Province 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《解剖学研究》2024年第6期540-545,共6页Anatomy Research

基　　金：湖北省自然科学基金项目(2022CFB669)。

摘　　要：目的探讨circFNDC3B调控巨噬细胞极化对口腔鳞状细胞癌细胞凋亡和GPNMB/CD44轴的作用机制。方法将过表达circFNDC3B质粒转染到M0型巨噬细胞中,分为con组和circFNDC3B组,RT-PCR检测巨噬细胞中CD16、TLR2、CD206和CD14相对表达量;将con组和circFNDC3B组巨噬细胞和OSCC细胞系SCC-25共培养,设为M0-con组和M0-circFNDC3B组;Transwell法检测细胞侵袭;流式细胞术检测细胞凋亡能力;蛋白质印迹法检测细胞中GPNMB和CD44蛋白表达。结果与con组相比,circFNDC3B组巨噬细胞中M1型巨噬细胞标志蛋白CD16、TLR2相对表达量均降低(P<0.01);M2型巨噬细胞标志蛋白CD206、CD14相对表达量均升高(P<0.01)。与M0-con组相比,M0-circFNDC3B组细胞侵袭数目及细胞中GPNMB和CD44蛋白表达明显增加,细胞凋亡率显著降低(P<0.01)。结论circFNDC3B可促进M2型巨噬细胞极化抑制口腔鳞状细胞癌细胞凋亡,其机制可能和激活GPNMB/CD44轴有关。Objective To investigate the mechanism of circFNDC3B-regulated macrophage polarization on apoptosis and GPNMB/CD44 axis in oral squamous cell carcinoma.Methods Transfect the overexpression circF-NDC3B plasmid into M0 macrophages and divide them into con group and circFNDC3B group.RT-PCR was used to detect the relative expression levels of CD16,TLR2,CD206,and CD14 in macrophages;Co culture macrophages from the con group and circFNDC3B group with OSCC cell line SCC-25,designated as M0 con group and M0 circF-NDC3B group;Transwell method for detecting cell invasion;Flow cytometry is used to detect the ability of cells to undergo apoptosis;Western blot was used to detect the expression of GPNMB and CD44 proteins in cells.Results Compared with the con group,the relative expression levels of M1 macrophage marker proteins CD16 and TLR2 in macrophages of the circFNDC3B group were reduced(P<0.01);The relative expression levels of M2 macrophage marker proteins CD206 and CD14 were both increased(P<0.01).Compared with the M0 con group,the M0 circF-NDC3B group showed a significant increase in the number of cell invasions and the expression of GPNMB and CD44 proteins in the cells,while the apoptosis rate was significantly reduced(P<0.05).Conclusion Inhibition of circF-NDC3B promotes M1-type macrophage polarization,which promotes apoptosis in oral squamous cell carcinoma cells and inhibits activation of the GPNMB/CD44 axis.

关 键 词：口腔鳞状细胞癌 来源于Ⅲ型纤维连接蛋白域蛋白3B 环状RNA 巨噬细胞极化 凋亡 GPNMB/CD44轴 

分 类 号：R739.8[医药卫生—肿瘤]