幽门螺杆菌对肝癌HepG2细胞C‐Met基因表达的影响  

Effect of Helicobacter pylori on expression Levels of C⁃Met gene in HepG2 cells

作　　者：陈光佩 黎伟康 戴佩玲 吴绍峰[2] 杨巧红[3] 许晓伍[1] 刘坤东 CHEN Guan-gpei;LI Wei-kang;DAI Pei-ling;WU Shao-feng;YaANG Qiao-hong;XU Xiao-wu;LIU Kun-dong(Department of Human Anatomy,School of Basic Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Center for Experimental Teaching,School of Basic Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Department of Pathology and Pathophysiology,School of Basic Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学基础医学院人体解剖学系,广东广州510006 [2]广州中医药大学基础医学院实验教学中心,广东广州510006 [3]广州中医药大学基础医学院病理学与病理生理学系,广东广州510006

出　　处：《解剖学研究》2024年第6期573-578,共6页Anatomy Research

基　　金：广东省自然科学基金面上项目(2020A1515010017);广东省中药新药研发重点实验室开放运行项目(2017B030314096);广州中医药大学海外留学归国人员“薪火计划”项目(XH20170101)。

摘　　要：目的研究幽门螺杆菌(Hp)对肝癌HepG2细胞原癌基因C-Met表达的影响。方法将Hp与HepG2细胞共同培养,设置空白对照组、Hp组、C-Met抑制剂空白组和C-Met抑制剂Hp组,每组设副孔5个,采用CCK8试剂盒检测HepG2细胞增殖活性,采用Western blot和qPCR方法检测C-Met原癌基因蛋白和mRNA表达水平。结果相较于空白对照组,Hp处理组HepG2细胞增殖活性升高且与时间呈正相关,而C-Met抑制剂可以抑制HepG2细胞的增殖活性。同时,Hp组HepG2细胞C-Met基因蛋白和mRNA表达水平均上调,C-Met抑制剂组则显著下调。相较于Hp共培养12 h,共培养24 h C-Met蛋白和mRNA表达水平均上调。结论Hp促进HepG2细胞的增殖可能与其提高原癌基因C-Met的蛋白及其mRNA表达水平有关,为后续更深入研究肝癌发生发展的复杂机制提供了理论依据。Objective To investigate the effects of Helicobacter pylori(Hp)on proto-oncogene C-Met in HepG2 cells.Methods Hp and HepG2 cells were co-cultured and the control group,HP group,c-Met inhibitor treatment group and c-Met inhibitor treatment Hp group were set up.Each group had 5 side holes.The proliferation activity of HepG2 cells was detected by CCK8 kit,and the expression level of C-Met protein and mRNA was detect-ed by Western blot and qPCR.Results Compared with the control group,the proliferation activity of HepG2 cells in Hp treatment group was increased and positive correlation with time,while the c-met inhibitor treatment group was down-regulated.Meanwhile,the expression levels of c-met protein and mRNA in Hp treatment group was ascended,while the c-met inhibitor treatment group decreased.Compared with 12 h,the expression levels of c-met protein and mRNA in Hp treatment for 24 h was up-regulated.Conclusion Hp can promote the proliferation of HepG2 cells,which may be related to the increase of the expression levels of proto-oncogene C-Met proteins and its mRNA in HepG2 cells,which provides a theoretical basis for further research on the complex mechanism of hepatocarcinogene-sis and development.

关 键 词：幽门螺杆菌 C‐Met 肝癌 HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤]