Celastrus orbiculatus Extract Inhibits Immune Inflammatory Thrombotic State of B-Lymphoma  

作　　者：ZHU Miao SHI Qing-qing NI Jun WU Wei SUN Xing SUN Mei XU Kai-lin LIU Yan-qing GU Jian GU Hao 

机构地区：[1]Department of Hematology,Clinical Medical College,Yangzhou University,Yangzhou,Jiangsu Province 225001,China [2]Yangzhou Institute of Hematology,Yangzhou,Jiangsu Province 225001,China [3]Department of Hematology,The Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu Province 221000,China [4]Department of Oncology,The Affiliated Hospitalof Yangzhou University,Yangzhou University,Yangzhou,Jiangsu Province 225001,China [5]Department of Neurology,The Affiliated Brain Hospital of Nanjing Medical University,Nanjing 210000,China

出　　处：《Chinese Journal of Integrative Medicine》2024年第11期1018-1026,共9页中国结合医学杂志（英文版）

基　　金：Supported by Chinese and Western Medicine Cooperation Project of Subei People's Hospital(No.ZXXTGG2022BO3);Jiangsu Graduate Scientific Research Innovation Program(No.SJCX22 1813)。

摘　　要：Objective:To investigate the inhibitory effect of Celastrus orbiculatus extracts(COE)on the proliferation of lymphoma cells and the immune regulation ability on inflammation and thrombophilia in vivo.Methods:The 38B9 lymphoma cells were treated with COE(160μg/mL)and CTX(25μmol/L).The apoptosis rate and cell cycle of each group were detected by flow cytometry.The secretion of inflammatory factors,including interleukin(IL)-6,IL-10,and tumor necrosis factorα(TNF-α),in cell supernatant was detected by enzyme-linked immunosorbent assay(ELISA).In vivo,BALB/c mice were subcutaneously injected with 38B9 lymphoma cells to establish lymphoma model.COE(3 mg·kg^(-1)·d^(-1))and CTX(40 mg·kg^(-1)·d^(-1))were administered to the model mice,respectively.The expression of plasma inflammatory factors(IL-6,IL-10 and TNF-α)and thrombus indexes,including D-dimer(D-D),von Willebrand factor(vWF)and tissue factor(TF),were detected by ELISA before tumor bearing(1 d),after tumor formation(14 d)and after intervention(21 d).PicoGreen dsDNA was used to detect the level of serum neutrophil extracellular traps(NETs).Flow cytometry was used to detect the expression of platelet activation marker calcium-dependent lectin-like receptor 2(CLEC-2).The tumor growth and survival of mice were recorded.Results:The 38B9 lymphoma cells were apoptotic after the intervention of COE and CTX.The ratio of G_(2)-M phase cells decreased in COE intervented cells compared with the control cells(P<0.05),and S phase cells decreased in CTX intervented cells(P<0.05).Also,the secretion level of IL-6 was significantly reduced after COE or CTX intervention(P<0.05),and IL-10 was significantly increased(P<0.05).Furthermore,the tumor mass was reduced,and the median survival time was longer in COE and CTX intervented tumor-bearing mice than in non-intervented mice.The significantly lower levels of TNF-α,IL-6,NETs,TF,DD and CLEC-2,as well as higher IL-10 were observed in COE and CTX treatment mice in comparision with the control mice(P<0.05).Conclusion:COE has a mil

关 键 词：Celastrus orbiculatus extract murine lymphoma cells tumor inflammation thrombotic state immune regulation proliferation inhibition LYMPHOMA 

分 类 号：R733.1[医药卫生—肿瘤]