YTHDF2在神经胶质瘤组织的表达及其对细胞恶性生物学行为的影响  

Expression of YTH domain family,member 2 in glioma tissues and its effect on malignant biological behavior

作　　者：孟磊[1] 胡朝帅 王向阳 申法政[1] Meng Lei;Hu Chaoshuai;Wang Xiangyang;Shen Fazheng(Department of Neurosurgery,the First Afiliated Hospital of Xinxiang Medical University,Weihui 453100,China)

机构地区：[1]新乡医学院第一附属医院神经外科,新乡453100

出　　处：《中华实验外科杂志》2024年第10期2252-2255,共4页Chinese Journal of Experimental Surgery

基　　金：2021年度河南省医学科技攻关计划联合共建项目(LHGJ20210515);2020年度新乡医学院第一附属医院青年培育基金项目(QN-2020-B07)。

摘　　要：目的:探讨YTHDF2在神经胶质瘤组织表达及其对神经胶质瘤细胞增殖和转移的影响。方法:选取2019年6月至2024年6月新乡医学院第一附属医院收治的90例神经胶质瘤组织和癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)和蛋白质免疫印迹分析癌旁组织和神经胶质瘤组织YTHDF2 mRNA和蛋白质的变化。采用对照短发卡RNA(shRNA)和YTHDF2 shRNA感染神经胶质瘤细胞U251和U373,构建U251/对照组、U251/YTHDF2 KD组,U373/对照组、U373/YTHDF2 KD组。采用细胞计数试剂盒(CCK-8)、细胞克隆形成实验分析细胞增殖能力;划痕实验和Transwell分析细胞的迁移和侵袭能力;蛋白质免疫印迹和荧光定量PCR分析YTHDF2目标基因表达变化。组间计量数据比较采用独立样本t检验。结果:神经胶质瘤组织中YTHDF2 mRNA表达水平(1.63±0.29)明显高于癌旁组织(0.95±0.21),差异有统计学意义(t=18.150,P<0.05)。神经胶质瘤组织中YTHDF2蛋白质表达水平(1.16±0.19)明显高于癌旁组织(0.50±0.15),差异有统计学意义(t=26.250,P<0.05)。U251/对照组和U373/对照组细胞吸光度值(1.63±0.29、1.63±0.29)明显高于U251/YTHDF2 KD组和U373/YTHDF2 KD组(1.25±0.09、1.22±0.10),差异有统计学意义(t=10.330、12.820,P<0.05)。U251/对照组和U373/对照组细胞形成率[(84.50±4.15)%、(85.85±5.12)%]明显高于U251/YTHDF2 KD组和U373/YTHDF2 KD组[(48.50±6.16)%、(60.35±5.82)%],差异有统计学意义(t=8.504、8.691,P<0.05)。U251/对照组和U373/对照组细胞划痕愈合率[(88.18±3.85)%、(87.01±4.15)%]明显高于U251/YTHDF2 KD组和U373/YTHDF2 KD组[(63.21±7.74)%、(65.07±4.34)%],差异有统计学意义(t=7.646、9.050,P<0.05)。U251/对照组和U373/对照组细胞侵袭数量[(138.86±13.69)、(126.29±12.18)个]明显高于U251/YTHDF2 KD组和U373/YTHDF2 KD组[(88.57±5.35)、(79.71±11.35)个],差异有统计学意义(t=9.672、7.401,P<0.05)。U251/对照组和U373/对照组细胞LHPP mRNA(1.00±0.09、0.82±0.07)�Objective:To investigate the expression of YTH domain family,member 2(YTHDF2)in glioma tissues and its effect on the proliferation and metastasis of glioma cells.Methods:Ninety of glioma tissue and adjacent tissue treated in the First Affiliated Hospital of Xinxiang Medical University from June 2019 to June 2024 were selected as the study objects,and the changes of YTHDF2 mRNA and protein in adjacent tissue and glioma tissue were analyzed by fluorescent quantitative polymerase chain reaction(PCR)and western blotting.Glioma cells U251 and U373 were infected with control short hairpin RNA(shRNA)and YTHDF2 shRNA to construct U251/control group,U251/YTHDF2 KD group,U373/control group,and U373/YTHDF2 KD group.Cell proliferation ability was analyzed by cell counting kit-8(CCK-8)and cell clonal formation assay.The migration and invasion ability of cells were analyzed by scratch assay and Transwell.The expression of YTHDF2 target gene was analyzed by Western blotting and quantitative PCR.Intergroup comparisons of quantitative data were made using independent sample t-tests.Results:The mRNA expression level of YTHDF2 in glioma tissues(1.63±0.29)was significantly higher than that in adjacent tissues(0.95±0.21),and the difference was statistically significant(t=18.150,P<0.05).The YTHDF2 protein expression level in glioma tissues(1.16±0.19)was significantly higher than that in adjacent tissues(0.50±0.15),and the difference was statistically significant(t=26.250,P<0.05).U251/control group and U373/control group(1.63±0.29,1.63±0.29)was significantly higher than that in U251/YTHDF2 KD and U373/YTHDF2 KD groups(1.25±0.09,1.22±0.10),the difference was statistically significant(t=10.330,12.820,P<0.05).The cell formation rate[(84.50±4.15)%,(85.85±5.12)%]in U251/control group and U373/control group was significantly higher than that in U251/YTHDF2 KD group and U373/YTHDF2 KD group[(48.50±6.16)%,(60.35±5.82)%],the difference was statistically significant(t=8.504,8.691,P<0.05).The cell scratch healing rate[(88.18±3.85)%,(

关 键 词：YTHDF2 神经胶质瘤 增殖 迁移 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]