粗茶皂素的纯化工艺优化  

Optimization of Purification Process for Crude Tea Saponin

作　　者：华军利[1] 黄家诚 敬思群 温金梅 HUA Junli;HUANG Jiacheng;JING Siqun;WEN Jinmei(Food College,Shaoguan University,Shaoguan,Guangdong 512005,China)

机构地区：[1]韶关学院食品学院,广东韶关512005

出　　处：《农产品加工》2024年第22期45-48,59,共5页Farm Products Processing

基　　金：2020年度韶关市科技计划项目社会发展方向高等院校科研项目(200811114531603);2021年度韶关学院校级自然科学类科研项目(SY2021KJ02)。

摘　　要：以粤北脱脂油茶粕为原材料,采用乙醇溶液提取粗茶皂素,再用氧化钙为沉淀剂、碳酸氢铵为转化剂进行纯化。通过单因素试验和正交试验考查了氧化钙添加量、沉淀反应时间、碳酸氢铵添加量和转化时间对茶皂素纯度的影响。结果表明,粗茶皂素纯化最佳工艺条件为氧化钙添加量为油茶粕质量的14%,沉淀时间为2 h,碳酸氢铵添加量为氧化钙质量的2倍,转化时间为1 h,在此条件下茶皂素纯度可达68.10%。Crude tea saponin was extracted from defatted Camellia oleifera meal in northern Guangdong by ethanol solution.Crude tea saponin was purified using calcium oxide as a precipitant and ammonium bicarbonate as a conversion agent.The effects of calcium oxide addition,precipitation reaction time,ammonium bicarbonate addition,and conversion time on the purity of tea saponin were investigated through single factor and orthogonal experiments.The experimental results showed that the optimal process conditions for purifying crude tea saponin were as follows:the amount of calcium oxide added was 14%of the mass of Camellia oleifera meal,the precipitation time was 2 h,the amount of ammonium bicarbonate added was twice the mass of calcium oxide,and the conversion time was 1 h.Under these conditions,the purity of tea saponins could reach 68.10%.

关 键 词：茶皂素 乙醇提取 沉淀纯化 

分 类 号：TQ423[化学工程]