绿色低共熔溶剂提取柚皮中多酚类物质的鉴定  

Identification of Polyphenols Extracted from Pomelo Peel byGreen Eutectic Solvent

作　　者：姜浩 郑晓青 程子贤 文芷懿 张沐棠 黎依琳 陈铎 梁一柱 陈椰娜 王宏 白卫东[1,2] JIANG Hao;ZHENG Xiaoqing;CHENG Zixian;WEN Zhiyi;ZHANG Mutang;LI Yilin;CHEN Duo;LIANG Yizhu;CHEN Ye'na;WANG Hong;BAI Weidong(Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology,Light Industry Food College,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225,China;Academy of Contemporary Agricultural Engineering Innovations,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225,China;Meizhou Dragon Fruit Industry Co.,Ltd.,Meizhou,Guangdong 514000,China;Guangdong Huazhou Agricultural Technology Extension Center,Huazhou,Guangdong 525100,China;Guangdong Hengsheng Weijia Food Industry Co.,Ltd.,Chaozhou,Guangdong 515700,China)

机构地区：[1]仲恺农业工程学院轻工食品学院,广东省岭南特色食品科学与技术重点实验室,广东广州510225 [2]仲恺农业工程学院现代农业工程创新研究院,广东广州510225 [3]广东省梅州市飞龙果业有限公司,广东梅州514000 [4]广东省化州市农业技术推广中心,广东化州525100 [5]广东亨盛维嘉食品工业有限公司,广东潮州515700

出　　处：《农产品加工》2024年第22期79-85,共7页Farm Products Processing

基　　金：广东省普通高校青年创新人才项目(2021KQNCX031);甜橙黄酮及其结构类似物对胰高血糖症的影响研究项目(KJCX2022010);广东省茂名市化州市现代农业产业园项目(2021-12);潮州特色果蔬在功能蛋白粉剂类食品应用关键技术研究及示范项目(202202ZD04);广州市科技计划项目一般项目(202201010268);化橘红黄酮提取工艺优化及其对胰高血糖症改善机制研究项目(KJCX2023011)。

摘　　要：在工业化生产中,一般采用传统有机溶剂对柚皮多酚类物质进行提取,以梅州柚柚皮和绿色溶剂离子液体-低共熔溶液为试验材料和提取溶剂,并运用高效液相色谱串联质谱对所提多酚进行鉴定分析。共检测出柚皮多酚提取物的819种物质,包括43种类黄酮、108种黄酮、78种酚类物质等,进而定量11种多酚化合物,含量从小到大依次为没食子酸、橙皮苷、槲皮素、异槲皮苷、儿茶素、表儿茶素、甜橙黄酮、异甜橙黄酮、异鼠李素、山奈酚、甲基橙皮苷的含量。其中,甲基橙皮苷和异鼠李糖的含量最高,异甜橙黄酮、甜橙黄酮次之,川陈皮素、儿茶素、表儿茶素、山奈酚、异槲皮苷含量较低。In industrial production,traditional organic solvents are generally used to extract polyphenols from pomelo peel.In this paper,Meizhou pomelo peel and green solvent ionic liquid-eutectic solution were used as test materials and extraction solvents,and the polyphenols were identified and analyzed by high performance liquid chromatography(HPLC)and mass spectrometry(MS).A total of 819 substances of polyphenol extract from grapefruit peel were detected,including 43 kinds of flavonoids,108 kinds of flavonoids,78 kinds of phenolic substances,and then 11 kinds of polyphenol compounds were quantified.From small to large,the contents were gallic acid,hesperidin,quercetin,isoquercetin,catechin,epicatechin,sinensetin,isosinensetin,isorhamnetin,kaempferol and methylhesperidin.The contents of methylhesperidin and isorhamnose were the highest,isosweet orange flavone and sweet orange flavone were the second,and the contents of tangerine,catechin,epicatechin,kaempferol and isoquercetin were lower.

关 键 词：柚子多酚 离子液体和低共熔溶剂 成分鉴定 液质联用 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]