SP免疫组化染色检测IGF-1及组织P53蛋白的表达在结直肠腺癌中意义  

The significance of SP immunohistochemical staining for detecting the expression of IGF-1 and tissue P53 in colorectal adenocarcinoma

作　　者：田彩云 田莉 王春玲 杜亚娟 杨利 TIAN Caiyun;TIAN Li;WANG Chunling;DU Yajuan;YANG Li(Department of Pathology,Urumqi Hospital of Traditional Chinese Medicine,Urumqi 830000,China)

机构地区：[1]乌鲁木齐市中医医院病理科,新疆乌鲁木齐830000

出　　处：《广州医药》2024年第11期1269-1274,共6页Guangzhou Medical Journal

摘　　要：目的探讨链霉菌抗生物素蛋白-过氧化物酶(SP)免疫组化染色检测胰岛素样生长因子-1(IGF-1)及组织P53蛋白的表达在结直肠腺癌中意义。方法选取乌鲁木齐市中医医院2020年1月—2023年12月收治的169例结直肠腺癌患者为恶性组,另选取我院同期收治的169例良性结直肠肿瘤患者为良性组。取手术或活检病理组织应用SP免疫组化染色检测2组肿瘤组织IGF-1及P53蛋白表达水平,对比良性组与恶性组间差异,分析不同临床分期、淋巴结转移情况及组织分化程度患者的IGF-1水平、IGF-1mRNA及P53蛋白阳性率。结果SP免疫组化染色检测IGF-1阳性率、P53蛋白阳性率,恶性组(72.78%、47.93%)均高于良性组(14.79%、6.51%),对比差异有统计学意义(χ^(2)=115.440、χ^(2)=73.180,P<0.05);Ⅳ期患者IGF-1阳性率(96.77%)及P53蛋白阳性率(77.42%)高于Ⅲ期(85.11%、63.83%)、Ⅱ期(62.69%、31.34%)及Ⅰ期患者(45.83%、25.00%),对比差异有统计学意义(χ^(2)=24.860,χ^(2)=28.000,P<0.05),各亚组两两比较差异无统计学意义(IGF-1阳性与阴性,Ⅰ期vsⅢ期,χ^(2)=12.110,P<0.001;Ⅰ期vsⅣ期,χ^(2)=16.060,P<0.001;Ⅱ期vsⅢ期,χ^(2)=6.880,P=0.009;P53蛋白阳性与阴性Ⅰ期vsⅢ期,χ^(2)=9.580,P<0.002;Ⅰ期vsⅣ期,χ^(2)=14.9990,P<0.001;Ⅱ期vsⅢ期,χ^(2)=11.790,P=0.001;);有淋巴结转移患者IGF-1阳性率(85.71%)及P53蛋白阳性率(71.43%)高于无淋巴结转移患者(14.79%、40.94%),对比差异有统计学意义(χ^(2)=4.720、11.740,P<0.05);低分化患者IGF-1阳性率(93.48%)及P53蛋白阳性率(71.74%)高于中分化患者(81.18%、54.12%)、高分化患者(52.63%、31.58%),对比差异有统计学意义(χ^(2)=21.250、13.510,P<0.05)。结论SP免疫组化染色检测提示结直肠腺癌患者IGF-1、P53蛋白阳性率高于良性肿瘤患者,且临床分期高、淋巴结转移与肿瘤组织低分化者的IGF-1、P53蛋白阳性率高,因此IGF-1、P53有望成为结直肠腺癌诊治的重要检测指标。Objective Exploring the significance of immunohistochemical staining with streptavidin-perosidase(SP)to detect the expression of insulin-like growth factor-1(IGF-1)and tissue P53 in colorectal adenocarcinoma.Methods A total of 169 patients with colorectal adenocarcinoma admitted to a hospital from January 2020 to December 2023 were selected and divided into the malignant group.Other 169 patients with benign colorectal tumors admitted to a hospital during the same period were selected and divided into the benign group.SP immunohistochemistry staining was used to detect and compare the expression levels of IGF-1 and P53 proteins in two groups of tumor tissues obtained in surgery or biopsy.And the IGF-1 levels,IGF-1 mRNA,and P53 positivity rates in patients with different clinical stages,lymph node metastasis,and tissue differentiation levels were compared.Results There was a significant difference in the positive rates of IGF-1mRNA and P53 detected by SP immunohistochemistry staining,and malignant group(72.78%,47.93%)were higher than the benign group(14.79%,6.51%),which were statistically significant(χ^(2)=115.440,73.180,P<0.05).The positive rates of IGF-1 and P53 were 96.77%and 77.42%in stage IV,which were higher than those in Stage III(85.11%,63.83%),II(62.69%,31.34%)and I(45.83%,25.00%),the differences were statistically significant(χ^(2)=24.860,28.000,P<0.05).The expression levels of IGF-1 and P53 in colorectal adenocarcinoma patients with different lymph node metastases showed significant differences,the positive rates of IGF-1(85.71%)and P53(71.43%)in patients with lymph node metastasis were higher than those without lymph node metastasis(14.79%,40.94%),and the differences were statistically significant(χ^(2)=4.720,11.740,P<0.05).There were significant differences in the expression levels of IGF-1 and P53 among patients with colorectal adenocarcinoma of different degrees of differentiation,the positive rates of IGF-1(93.48%)and P53(71.74%)were significantly higher than those of moderately differentiated(81

关 键 词：免疫组化染色 胰岛素样生长因子-1 P53 结直肠腺癌 

分 类 号：R735.3[医药卫生—肿瘤]