甲醇微生物转化最新进展  

Advances in microbial bioconversion of methanol

作　　者：蔡鹏 吴晓燕 解林峰 申益维 高琳惠 周雍进[1,2] Peng Cai;Xiaoyan Wu;Linfeng Xie;Yiwei Shen;Linhui Gao;Yongjin Zhou(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院大学,北京100049

出　　处：《中国科学：化学》2024年第11期2199-2218,共20页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金青年基金(编号:22308352);中国博士后科学基金面上项目(编号:2023M733450)资助。

摘　　要：甲醇生物转化是CO_(2)转化和可再生能源利用的有效方式,是低碳生物转化研究领域的热点.然而微生物在甲醇代谢模式下面临甲醇生物毒性大、甲醇耐受性低、代谢适配性差等诸多挑战,导致了较低的生物合成效率.本文综述了甲醇微生物转化近年来的研究现状,重点总结了天然甲基营养型菌株与人工甲基营养型菌株的代谢途径改造与设计,甲醇代谢过程的理性改造与进化工程策略,甲醇发酵工艺现状等内容.最后,探讨了当前甲醇生物转化面临的挑战,并为甲醇生物转化的产业化提出了可行的发展方向.Methanol bioconversion represents a critical approach for addressing the global climate crisis and achieving sustainable low-carbon production.However,microorganisms encounter significant challenges under methanol metabolism,including biological toxicity,low methanol tolerance,and tight metabolic adaptation,resulting in reduced cell growth and chemical production.This review provides an overview of recent advancements in engineering microbial conversion of methanol,with a particular emphasis on reconstructing and designing of metabolic pathways in both natural and synthetic methylotrophs.It also discussed rational redesign and adaptive evolution strategies in regulating methanol metabolism,alongside the current state of methanol fermentation processes.Finally,we discussed the current challenges of methanol bioconversion and put forward the future direction for the industrialization of methanol bioconversion.

关 键 词：甲醇生物转化 甲醇脱氢酶 甲醛同化 甲醇细胞工厂 适应性进化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]