上调miR-124-3p表达增加U-87 MG人胶质母细胞瘤细胞的放疗敏感性  

Upregulation of miR-124-3p expression increases the radiosensitivity of U-87 MG human glioblastoma cells

作　　者：麦麦提依明·托合提 张诚[1] 黄啸元 高峰[1] 帕热哈提江·依孜木 阿卜杜喀迪尔·牙森 董军[1] 杨小朋[1] 吴永刚[1] MAIMAITIYIMING Tuoheti;ZHANG Cheng;HUANG Xiao-yuan;GAO Feng;PAERHATIJIANG Yizimu;ABUDUKADIER Yasen;DONG Jun;YANG Xiao-peng;WU Yong-gang(Department of Neurosurgery,The People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院神经外科,830001乌鲁木齐

出　　处：《中国临床神经外科杂志》2024年第7期422-426,共5页Chinese Journal of Clinical Neurosurgery

基　　金：新疆维吾尔自治区自然科学基金(2022D01C625,2019D01C107,2017D01C146)。

摘　　要：目的探讨miR-124-3p表达水平对U-87 MG人胶质母细胞瘤细胞(U87细胞)放疗敏感性的影响。方法体外培养U87细胞,利用脂质体载体转染技术转染hsa-miR-124-3p mimics或hsa-miR-124-3p inhibitor质粒调控miR-124-3p表达;转染24 h后,取生长状态良好的U87细胞,在室温下用西门子Primus-M型直线加速器进行照射(16 Gy)模拟放疗;实时荧光定量PCR检测细胞miR-124-3p表达水平,CCK-8法检测细胞增殖能力。结果转染hsa-miR-124-3p mimics质粒后,细胞miR-124-3p表达水平明显上调(P<0.05);转染hsa-miR-124-3p inhibitoR质粒后,细胞miR-124-3p表达水平明显下调(P<0.05)。上调miR124-3p表达,U87细胞增殖活性明显降低(P<0.05);下调miR-124-3p表达,U-87细胞增殖活性明显增高(P<0.05)。上调miR124-3p表达联合放疗,U87细胞增殖活性进一步明显降低(P<0.05);下调miR-124-3p表达联合放疗,U87细胞增殖活性明显降低,但仍明显高于未给予任何处理的U87细胞的增殖活性(P<0.05)。结论上调miR-124-3p表达明显抑制U87细胞的增殖活性,并且显著增加U87细胞的放疗敏感性。Objective To explore the effect of miR-124-3p expression on the radiosensitivity of U-87 MG human glioblastoma cells(U87 cells).Methods U87 cells were cultured in vitro.The expression of miR-124-3p was regulated by transfecting hsa-miR124-3p mimics or hsa-miR-124-3p inhibitor plasmids using liposome vector transfection technology.After 24 hours of transfection,U87 cells in good growth condition were irradiated at room temperature using a Siemens Primus-M lineaRaccelerator (16 Gy)to simulate radiotherapy.The expression level of miR-124-3p in U87 cells was detected by real-time fluorescence quantitative PCR,and the proliferation ability of U87 cells was detected by the CCK-8 method.Results After transfection with hsa-miR-124-3p mimics plasmids,the expression level of miR-124-3p in U87 cells was significantly upregulated(P<0.05);after transfection with hsa-miR-124-3p inhibitor plasmids,the expression level of miR-124-3p in U87 cells was significantly downregulated(P<0.05).Upregulating the expression of miR-124-3p significantly reduced the proliferation activity of U87 cells(P<0.05);downregulating the expression of miR124-3p significantly increased the proliferation activity of U87 cells(P<0.05).The combination of upregulating miR-124-3p expression and radiotherapy further significantly reduced the proliferation activity of U87 cells(P<0.05);the combination of downregulating miR124-3p expression and radiotherapy significantly reduced the proliferation activity of U87 cells,but was still significantly higher than the proliferation activity of U87 cells without any treatment(P<0.05).Conclusions Upregulating the expression of miR-124-3p significantly inhibits the proliferation activity of U87 cells and significantly increases theiRradiosensitivity.

关 键 词：胶质母细胞瘤 U87 MG细胞 miR-124-3p 细胞增殖 放疗敏感性 

分 类 号：R739.41[医药卫生—肿瘤]