采摘时期对沙枣叶茶黄酮与多酚含量影响的研究  

Study on Effect of Picking Time on Contents of Flavone and Polyphenol in Elaeagnus angustifolia Leaf Tea

作　　者：张芳 张欣[1,2,3] 逯露露 白喜龙 贝盏临 Zhang Fang;Zhang Xin;Lu Loulou;Bai Xilong;Bei Zhanlin(College of Biological Science and Engineering,Beifang Ethnic University,Yinchuan,Ningxia 750021;State Ethnic Affairs Commission Key Laboratory of Ecological Protection in Agro-Pastoral Intercropping Area of Yellow River Basin,Yinchuan,Ningxia 750021;Ningxia Economic Forest Genetic Improvement Innovation Team,Yinchuan,Ningxia 750021)

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021 [2]国家民委黄河流域农牧交错区生态保护重点实验室,宁夏银川750021 [3]宁夏经济林遗传改良创新团队,宁夏银川750021

出　　处：《宁夏农林科技》2024年第9期41-50,共10页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：宁夏回族自治区级大学生创新创业训练计划项目(S202311407001、S202411407080);北方民族大学校级创新项目(2024-XJ-SK-005);北方民族大学白芨滩项目(SKBJT202204);宁夏经济林遗传改良创新团队(2022QCXTD04)。

摘　　要：茶叶因富含多种对人体有益的活性化合物而被全球广泛消费。茶叶中的黄酮类和多酚类化合物常被作为衡量茶叶功效的重要指标之一,对于新开发的茶叶产品,测量其中的黄酮和多酚含量有助于评估该茶叶的功效。研究以新开发的沙枣叶茶为对象,通过设置正交试验优化黄酮与多酚的提取条件、采用单因素试验确定最佳的反应试剂用量与时间,探究不同采摘时期对沙枣叶茶中黄酮与多酚含量的影响。结果显示,5月中旬、9月中旬、9月下旬采摘的沙枣叶茶其总黄酮含量分别为1.086、2.534、1.219 mg·g^(-1),总酚含量分别为37.330、34.094、17.914mg·g^(-1)。方差分析显示,不同采摘时期沙枣叶茶间的黄酮含量存在极显著差异(P<0.001)、多酚含量存在显著差异(P<0.05)。研究表明,9月中旬采摘的沙枣叶具有最高的黄酮、多酚含量,是制备沙枣叶茶的理想采摘时期。研究结果为沙枣叶茶的生产提供了科学依据,对农业生产具有实际的指导意义。Tea is widely consumed worldwide because it contains a variety of active compounds that are beneficial to the human body.Flavonoids and polyphenols in tea are often used as one of the important indicators to measure the efficacy of tea.For newly developed tea products,measuring the content of flavonoids and polyphenols is helpful to evaluate the efficacy of the tea.Taking the newly developed Elaeagnus angustifolia leaf tea as the object,orthogonal test was set to optimize the extraction conditions of flavonoids and polyphenols,and single factor test was used to determine the best reaction reagent dosage and time,so as to explore the effects of different picking periods on the contents of flavonoids and polyphenols in Elaeagnus angustifolia leaf tea.The results showed that the total flavonoid content and total phenol content of the leaf tea picked in mid-May,mid-September and late September were 1.086,2.534 and 1.219 mg·g^(-1),respectively,and 37.330,34.094 and 17.914 mg·g^(-1),respectively.The analysis of variance showed that there were significant differences in the contents of flavonoids and polyphenols between different picking periods(P<0.001).The results showed that the leaf tea picked in mid-September had the highest flavonoid and polyphenol content,which was the ideal picking period for preparing the leaf tea.The results provided a scientific basis for the production of Elaeagnus angustifolia leaf tea and had practical guiding significance for agricultural production.

关 键 词：沙枣叶茶 黄酮 多酚 采摘时间 

分 类 号：S793.9[农业科学—林木遗传育种]