响应面法优化牛大力茎总黄酮提取工艺  

Optimization in Extraction Process of Total Flavonoids from Millettia speciosa Champ.Stem by Response Surface Methodology

作　　者：梁子昌 陈明 李大宁 张如华 张剑光 LIANG Zichang;CHEN Ming;LI Daning;ZHANG Ruhua;ZHANG Jianguang(Qinzhou Provincial Health School,Qinzhou 535000,China;Department of Pharmaceutical and Chemical Engineering,Guangxi Nanning Technician College,Nanning 530007,China;The Second People′s Hospital of Qinzhou,Qinzhou 535000,China)

机构地区：[1]钦州市卫生学校,广西钦州535000 [2]广西南宁技师学院医药化工系,广西南宁530007 [3]钦州市第二人民医院,广西钦州535000

出　　处：《化学与生物工程》2024年第12期30-35,共6页Chemistry & Bioengineering

基　　金：国家重点研发计划项目(2019YFC1712503);钦州市科学技术局基础研究项目(20198509)。

摘　　要：以牛大力茎为研究对象,采用超声辅助乙醇法提取总黄酮。通过单因素实验考察了乙醇体积分数、液料比、提取时间、提取温度对牛大力茎总黄酮提取量的影响,并在此基础上,采用响应面法进一步优化总黄酮提取工艺。结果表明,在乙醇体积分数为70%、液料比为41∶1(mL∶g)、提取时间为42 min、提取温度为62℃的最优条件下,牛大力茎总黄酮提取量为16.06 mg·g^(-1),与预测值(16.23 mg·g^(-1))接近。该优化提取工艺操作简单,提取量高,为牛大力茎的利用及产品研发提供了依据。Taking Millettia speciosa Champ.stem as a research object,we extracted total flavonoids by ultrasonic-assisted ethanol extraction method.Moreover,we investigated the effects of ethanol volume fraction,liquid-solid ratio,extraction time,and extraction temperature on the extraction amount of total flavonoids from Millettia speciosa Champ.stem by single-factor experiments.Furthermore,we optimized the extraction process of total flavonoids by response surface methodologies.The results show that the extraction amount of total flavonoids from Millettia speciosa Champ.stem is 16.06 mg·g^(-1),which is close to the predicted value(16.23 mg·g^(-1))under the optimal conditions as follows:the ethanol volume fraction of 70%,the liquid-solid ratio of 41∶1(mL∶g),the extraction time of 42 min,and the extraction temperature of 62℃.The optimized extraction process is simple to operate,and has a high extraction amount,providing a basis for the utilization and product research and development of Millettia speciosa Champ.stem.

关 键 词：牛大力 超声辅助提取 总黄酮 响应面法 

分 类 号：R284.2[医药卫生—中药学]