活性炭对极大节旋藻来源藻蓝蛋白和蛋白质的吸附性能和机制研究  

Study on Adsorption Capacity and Mechanism of Activated Charcoal on Arthrospira maxima -derived C-phycocyanin and Proteins

作　　者：白仁奥 BAI Ren-ao(College of Building and Ecology,Shantou Polytechnic,Shantou,Guangdong 515078)

机构地区：[1]汕头职业技术学院建设生态学院,广东汕头515078

出　　处：《安徽农业科学》2024年第23期1-6,共6页Journal of Anhui Agricultural Sciences

基　　金：广东省高校特色创新项目(自然科学)(2023KTSCX332);汕头职业技术学院第二批高层次人才科研启动基金项目(SZK2022G18)。

摘　　要：通过等温吸附、吸附动力学和吸附热力学试验,揭示活性炭对C-PC和总蛋白质的吸附特性和作用机制。结果显示:同样条件下,活性炭去除杂蛋白质的效率更高,经过活性炭纯化后,C-PC占总蛋白质的比例从31.2%增加到51.5%;活性炭对C-PC和总蛋白质的最大吸附量分别为10.5和88.5 mg/g;活性炭对C-PC的吸附为单分子层化学吸附,符合Langmuir等温吸附模型,而对总蛋白质的吸附为多层级的吸附,符合Freundlich等温线模型。The isotherms,adsorption kinetics,and thermodynamics analyses were employed to elucidate the adsorption properties and mechanisms of action of activated charcoal on C-PC and total proteins.The results revealed that:under the same conditions,activated charcoal was more efficient in removing heteroproteins,and after purification with activated charcoal,the proportion of C-PC to the total protein increased from 31.2%to 51.5%;the maximum adsorption of C-PC and total protein by activated charcoal was 10.5 mg/g and 88.5 mg/g,respectively;the adsorption of C-PC by activated charcoal was a monomolecular layer chemisorption,consistent with the Langmuir isothermal adsorption model,whereas the adsorption of total proteins of activated charcoal was a multilayer adsorption,consistent with the Freundlich isotherm model.

关 键 词：螺旋藻 藻蓝蛋白 蛋白质提取 活性炭 吸附等温曲线 

分 类 号：Q946[生物学—植物学]