濒危赤水凤仙花叶绿体基因组特征及系统发育分析  

Characterization and phylogenetic analysis of the chloroplast genome of endangered Impatiens chishuiensis

作　　者：兰文香 莫清 黄海泉[1,2] 黄美娟[1,2] LAN Wenxiang;MO Qing;HUANG Haiquan;HUANG Meijuan(College of Landscape Architecture and Horticulture,Southwest Forestry University,Kunming 650224,China;Southwest Landscape Architecture Engineering Technology Research Center of National Forestry and Grassland Bureau,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院,昆明650224 [2]西南林业大学国家林业和草原局西南风景园林工程技术研究中心,昆明650224

出　　处：《西北植物学报》2024年第12期1927-1936,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(32060364,32060366);云南省重大科技专项(202102AE090052);云南省园林植物遗传改良与高效繁育博士生导师团队资助项目。

摘　　要：【目的】研究赤水凤仙花的叶绿体基因组结构特征与系统发育位置,以期为赤水凤仙花植物种质资源保护、遗传多样性和系统发育研究提供科学依据。【方法】以中国特有种——赤水凤仙花为材料,基于赤水凤仙花叶绿体基因组序列,利用生物信息学软件对叶绿体基因组进行组装,注释,基因特征、序列重复和系统发育分析。【结果】(1)赤水凤仙花叶绿体基因组呈典型的四分体结构,总GC含量为37%,长152892 bp;共编码120个基因,包括95个蛋白质编码基因、8个rRNA基因和17个tRNA基因。(2)共检测到87个SSR序列,以A、T组成为主;检测到20327个密码子,其中亮氨酸(Leu)最多,色氨酸(Trp)最少。(3)凤仙花属分为2个亚属,为棒凤仙花亚属与凤仙花亚属,赤水凤仙花为棒凤仙花亚属,且与太子凤仙花亲缘关系最近。【结论】赤水凤仙花为典型的四分体结构,SSR序列以A/T单碱基为主;系统发育分析结果将其归为棒凤仙花亚属。[Objective]The chloroplast genome structure and phylogenetic location of Impatiens chishuiensis were studied in order to provide scientific basis for the conservation of plant germplasm resources,genetic diversity,and phylogeny of I.chishuiensis.[Methods]Based on the chloroplast genome sequence of I.chishuiensis,an endemic species in China,the assembly,annotation,gene characterization,sequence repetition and phylogenetic analysis of chloroplast genome were performed by bioinformatic softwares.[Results](1)The chloroplast genome of I.chishuiensis has a typical tetrameric structure with a total GC content of 37%and a length of 152892 bp.It encodes a total of 120 genes,including 95 protein-coding genes,8 rRNA genes,and 17 tRNA genes.(2)A total of 87 SSR sequences was detected.20327 codons were detected,with leucine(Leu)being the most abundant and tryptophan(Trp)being the least.(3)Impatiens was divided into two subgenera,including subg.Clavicarpa and subg.Impatiens.I.chishuiensis is a species of subg.Clavicarpa and is the closest with I.alpicola.[Conclusion]I.chishuiensis has a typical tetrad structure,and the SSR sequence is mainly formed by A/T single base.Phylogenetic analyses place it in the subg.Clavicarpa.

关 键 词：赤水凤仙花 叶绿体基因组 序列结构与特征分析 系统发育分析 

分 类 号：Q943[生物学—植物学] R932[医药卫生—生药学]