基于网络药理学和动物实验探究芍药苷调节AMPK/Nrf2通路改善大鼠急性脑梗死  

Exploring the improvement of acute cerebral infarction in rats by regulating the AMPK/Nrf2 pathway with paeoniflorin based on network pharmacology and animal experiments

作　　者：薛娣[1] 牛森 王方[1] 侯艳丽[1] 

机构地区：[1]南阳医学高等专科学校,河南南阳473000

出　　处：《医学前沿》2024年第14期249-250,共2页Frontiers of Medicine

基　　金：2023年度河南省高等学校重点科研项目(23B310014)。

摘　　要：目的探讨芍药苷调节腺苷酸活化蛋白激酶信号通路对急性脑梗死大鼠的神经保护作用。方法检索Swiss Target Prediction获得芍药苷活性靶点,利用GeneCards、OMIM、TTD数据库预测急性脑梗死靶点。交集靶点导入STRING数据库和DAVID数据库分别绘制PPI网络图和KEGG通路富集。通过线栓闭塞大鼠大脑中动脉建立急性脑梗死模型,评测大鼠学习记忆功能、脑梗死面积、海马神经元凋亡、过氧化氢酶、活性氧含量和AMPK信号通路。结果PI3K-Akt、FoxO、HIF、AMPK、NF-kB等通路与急性脑梗死关系密切。与模型组相比,治疗组大鼠脑组织CAT含量、p-AMPK、Nrf2均显著升高,ROS含量、脑梗死面积、海马神经元凋亡显著降低(P<0.05)。结论芍药苷可通过激活AMPK信号通路发挥多靶点作用,降低氧化应激,减轻脑组织梗死和神经元凋亡发挥神经保护作用。Objective:Exploring the neuroprotective effects of paeoniflorin on acute cerebral infarction rats by regulating the adenylate activated protein kinase signaling pathway.Method:Retrieve the Swiss Target Prediction to obtain the active target of paeoniflorin,and use GeneCards,OMIM,and TTD databases to predict the target of acute cerebral infarction.Import the intersection targets into STRING database and DAVID database to draw PPI network diagrams and KEGG pathway enrichment,respectively.An acute cerebral infarction model was established in rats by occluding the middle cerebral artery with a thread,and the learning and memory function,infarct area,hippocampal neuron apoptosis,catalase,reactive oxygen species content,and AMPK signaling pathway were evaluated.Results:The PI3K-Akt,FoxO,HIF,AMPK,NF-kB pathways are closely related to acute cerebral infarction.Compared with the model group,the CAT content,p-AMPK,and Nrf2 of the treatment group rats were significantly increased,while the ROS content,infarct area,and hippocampal neuron apoptosis were significantly reduced.Conclusion:Paeoniflorin can exert multi-target effects by activating the AMPK signaling pathway,reducing oxidative stress,alleviating brain tissue infarction and neuronal apoptosis,and exerting neuroprotective effects.

关 键 词：芍药苷 网络药理学 腺苷酸活化蛋白激酶 急性脑梗死 

分 类 号：R74[医药卫生—神经病学与精神病学]