三阴性乳腺癌组织中miR-136-5p和FAM83H的表达及与临床病理特征和预后的相关性  

Expression of miR-136-5p and FAM83H in triple negative breast cancer and their correlation with clinicopathological characteristics and prognosis

作　　者：苗雅云 张冠男[1] 吴海滨[1] 王文胜[1] MIAO Yayun;ZHANG Guannan;WU Haibin;WANG Wensheng(Department of Breast and Thyroid Surgery,The First Affiliated Hospital of Henan University,Kaifeng,Henan 475004,China)

机构地区：[1]河南大学第一附属医院乳腺甲状腺外科,河南开封475004

出　　处：《社区医学杂志》2024年第19期656-662,共7页Journal Of Community Medicine

基　　金：河南省2021年科技发展计划(212102310164)。

摘　　要：目的探索三阴性乳腺癌(TNBC)组织中微小RNA-136-5p(miR-136-5p)和83序列相似家族成员H(FAM83H)的表达情况及其与患者临床病理特征和预后的相关性。方法选取2015-01-30-2018-06-30河南大学第一附属医院进行手术治疗的172例TNBC患者为研究对象,采用实时荧光定量聚合酶链式反应(RTFQ-PCR)检测其癌组织与癌旁组织中miR-136-5p、FAM83H的表达情况。采用Pearson法分析TNBC患者癌组织miR-136-5p与FAM83H表达水平的相关性。采用Kaplan-Meier生存曲线分析TNBC患者癌组织miR-136-5p与FAM83H表达水平与患者预后的关系。采用Cox回归模型分析TNBC患者预后不良影响因素。结果TNBC患者癌组织miR-136-5p水平为0.81±0.14,低于癌旁组织的1.02±0.21,差异有统计学意义,t=10.912,P<0.001;FAM83H水平为1.39±0.27,高于癌旁组织的0.99±0.17,差异有统计学意义,t=16.442,P<0.001。TNBC患者癌组织miR-136-5p与FAM83H表达水平呈显著负相关,r=-0.652,P<0.001。有淋巴结转移的TNBC患者癌组织miR-136-5p水平为0.72±0.09,低于无淋巴结转移的0.87±0.17,FAM83H水平为1.51±0.38,高于无淋巴结转移的1.31±0.20,差异有统计学意义,t值分别为6.685、4.502,均P<0.05;有血管侵犯的TNBC患者癌组织miR-136-5p水平为0.71±0.11,低于无血管侵犯的0.83±0.15,FAM83H水平为1.54±0.33,高于无血管侵犯的1.36±0.26,差异有统计学意义,t值分别为4.087、3.240,均P<0.05。172例TNBC患者5年失访5例,余下167例的预后不良发生率为24.55%,miR-136-5p高表达组预后不良发生率(15.79%)低于低表达组(31.87%),差异有统计学意义,χ^(2)=6.252,P=0.012;FAM83H高表达组预后不良发生率(35.00%)高于FAM83H低表达组(14.94%),差异有统计学意义,χ^(2)=11.522,P<0.001。多因素Cox回归分析显示,淋巴结转移、血管侵犯、癌组织FAM83H表达是导致TNBC患者预后不良的危险因素,癌组织miR-136-5p表达为保护因素,均P<0.05。结论TNBC患者癌组织miR-136-5p表达下调,FAM83H表达�Objective To investigate the expression of microRNA-136-5p(miR-136-5p)and family with sequence similarity83 member H(FAM83H)in triple negative breast cancer(TNBC)tissues and their correlation with clinicopathological characteristics and prognosis.Methods A total of 172 patients with TNBC who underwent surgical treatment at the First Affiliated Hospital of Henan University from January 30,2015 to June 30,2018 were selected as the study subjects.Real-time fluorescence quantitative polymerase chain reaction(RTFQ"PCR)was applied to detect the expression of miR-136-5p and FAM83H in cancerous and paracancerous tissues.Pearson method was applied to analyze the correlation between miR-136-5p and FAM83H expression levels in cancer tissue of TNBC patients.Kaplan-Meier survival curve was applied to analyze the relationship between the expression levels of miR-136-5p and FAM83H in cancer tissue of TNBC patients and their prognosis.Cox regression model was applied to analyze the factors affecting the poor prognosis of TNBC patients.Results The level of miR-136-5p in cancer tissues(0.81±0.14)of TNBC patients was lower than that in adjacent tissues(1.02±0.21),and the difference was statistically significant,t=10.912,P<0.001;while the level of FAM83H(1.39±0.27)was higher than that in adjacent tissues(0.99±0.17),and the difference was statistically significant,t=16.442,P<0.001.There was a obvious negative correlation between miR-136-5p and FAM83H expression levels in cancer tissue of TNBC patients,r=-0.652;P<0.001.The level of miR-136-5p in cancer tissues(0.72±0.09)of TNBC patients with lymph node metastasis was lower than that without lymph node metastasis(0.87±0.17),while the level of FAM83H(1.51±0.38)was higher than that in patients without lymph node metastasis(1.31±0.20),and the difference was statistically significant,t values were 6.685,4.502,all P<0.05,respectively;the level of miR-136-5p in cancer tissues(0.71±0.11)of TNBC patients with vascular invasion was lower than that in patients without vascular invasion(

关 键 词：三阴性乳腺癌 微小RNA-136-5p 83序列相似家族成员H 临床病理特征 预后 

分 类 号：R737.9[医药卫生—肿瘤]