枳椇果梗多糖对酒精诱导sh-sy5y细胞铁死亡的改善作用  

Ameliorative Effect of Hovenia dulcis Peduncle Polysaccharides on Alcohol-Induced Ferroptosis in sh-sy5y Cells

作　　者：张玉超 袁子飞 刘良禹 朱思洁 李玉勇 任文 雷豪 刘旭东 ZHANG Yuchao;YUAN Zifei;LIU Liangyu;ZHU Sijie;LI Yuyong;REN Wen;LEI Hao;LIU Xudong(Department of Brewing Engineering,Moutai Institute,Renhuai 564507,China;Department of Food Science and Engineering,Moutai Institute,Renhuai 564507,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266071,China)

机构地区：[1]茅台学院酿酒工程系,贵州仁怀564507 [2]茅台学院食品科学与工程系,贵州仁怀564507 [3]中国海洋大学食品科学与工程学院,山东青岛266071

出　　处：《食品科技》2024年第10期216-223,共8页Food Science and Technology

基　　金：贵州省基础研究(自然科学)计划项目(黔科合基础-ZK[2022]一般541、黔科合基础-ZK[2022]一般542);遵义市科学技术局、茅台学院市校联合科技研发资金项目(遵市科合HZ字(2021)330号,遵市科合HZ字(2022)169号);茅台学院高层次人才科研启动经费项目(mygccrc[2022]010);贵州省大学生创新创业训练计划项目(S202214625019,S202214625021)。

摘　　要：为探究枳椇果梗多糖(Hovenia dulcis fruit peduncle polysaccharides,HDPs)对酒精诱导shsy5y细胞铁死亡的改善作用,该文章对sh-sy5y细胞进行不同浓度酒精及HDPs处理,对处理后细胞的活性氧(Reactive oxygen species,ROS)、丙二醛(Malondialdehyde,MDA)、4-羟基壬烯醛(4-Hydroxynonenal,4-HNE)、还原型谷胱甘肽(Glutathione,GSH)、Fe^(2+)含量及谷胱甘肽过氧化物酶(Glutathione peroxidase 4,GPX-4)活性进行测定,明确酒精对sh-sy5y细胞铁死亡的诱导及HDPs对铁死亡的改善作用;同时对细胞内Warburg效应关键指标丙酮酸脱氢酶激酶1(Pyruvate dehydrogenase kinase isozyme 1,PDK-1)、乳酸脱氢酶A(Lactate dehydrogenase A,LDHA)和乳酸表达量进行测定,探究酒精诱导sh-sy5y细胞铁死亡及HDPs对铁死亡改善作用的潜在机制。结果显示,酒精暴露后sh-sy5y细胞内ROS、4-HNE、Fe^(2+)和MDA含量上升,细胞活力、GSH含量及GPX-4活性下降,同时PDK-1、LDHA和乳酸表达量增加;而HDPs可以显著抑制上述变化。结果表明,酒精暴露可诱导sh-sy5y细胞铁死亡,HDPs对铁死亡有改善作用;该抑制作用与下调铁死亡过程中的Warburg效应水平相关。研究结果可为明确HDPs对酒精性神经损伤的改善作用提供参考,对于促进枳椇资源的开发利用具有一定意义。In order to investigate the ameliorative effect of Hovenia dulcis fruit peduncle polysaccharides(HDPs)on alcohol-induced ferroptosis in sh-sy5y cells,sh-sy5y cells were treated with different concentrations of alcohol and HDPs.The contents of reactive oxygen species(ROS),malondialdehyde(MDA),4-hydroxynonenal(4-HNE),glutathione(GSH),Fe^(2+),and glutathione peroxidase 4(GPX-4)activity were measured to clarify the induction of ferroptosis by alcohol in sh-sy5y cells and the ameliorative effect of HDPs.At the same time,the key indicators of the Warburg effect,pyruvate dehydrogenase kinase isozyme 1(PDK-1),lactate dehydrogenase A(LDHA),and lactate expression were measured to explore the potential mechanism of alcohol-induced ferroptosis in sh-sy5y cells and the ameliorative effect of HDPs.The results showed that after exposure to alcohol,the contents of ROS,4-HNE,Fe^(2+),and MDA in sh-sy5y cells increased;the cell viability,GSH level,and GPX-4 activity decreased;at the same time,the expression levels of PDK-1,LDHA,and lactate increased,while HDPs could significantly inhibit these changes.The results indicated that alcohol exposure could induce ferroptosis in sh-sy5y cells and HDPs could ameliorate the ferroptosis.The ameliorative effect related to down-regulating the Warburg effect during ferroptosis.The research results provide a reference for clarifying the ameliorative effect of HDPs on alcohol-induced nerve injury,which is significance for promoting the development and utilization of Hovenia dulcis resources.

关 键 词：枳椇果梗多糖 酒精 SH-SY5Y细胞 铁死亡 改善作用 

分 类 号：TS201.23[轻工技术与工程—食品科学]