补播和施肥对高寒退化草地根际细菌群落的影响  

Effects of Reseeding and Fertilization on Bacterial Communities in Rhizosphere Soil ofAlpine Degraded Grassland

作　　者：于皓 刘琦 邓晔[2] 顾松松 芦光新[3] 周学丽 YU Hao;LIU Qi;DENG Ye;GU Song-song;LU Guang-xin;ZHOU Xue-li(College of Environmental Science and Engineering,Liaoning Technical University,Fuxin 123000,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Grassland Improvement Experimental Station,Gonghe 813099,China)

机构地区：[1]辽宁工程技术大学环境科学与工程学院,阜新123000 [2]中国科学院生态环境研究中心,北京100085 [3]青海大学农牧学院,西宁810016 [4]青海省草原改良试验站,共和813099

出　　处：《环境科学》2024年第12期7350-7357,共8页Environmental Science

基　　金：辽宁省应用基础研究计划项目(2022JH2/101300196);中国科学院环境生物技术重点实验室开放课题项目(kf2020005);青海省重点研发与转化计划项目(2022-SF-147);国家自然科学基金区域创新发展联合基金项目(U23A2043)。

摘　　要：对退化草地进行补播和施肥是青藏高原地区常见的生态恢复措施,但其对草地根际细菌群落的影响尚不清楚.采用高通量测序技术,系统地对采取补播和施肥管理的退化高寒草地根际土壤细菌群落的特征进行研究分析.结果表明,施肥处理后Shannon指数和Simpson指数显著降低,施肥+补播处理对群落结构影响最明显.放线菌门(Actinomycetota)、变形菌门(Pseudomonadota)和酸杆菌门(Acidobacteriota)为根际细菌群落的主要优势菌门.补播、施肥和施肥+补播处理后细菌群落网络复杂程度下降、稳定性增加,且网络以正相关为主.假单胞菌门(Pseudomonadota)、放线菌门(Actinomycetota)和酸杆菌门(Acidobacteriota)为样地关键物种. Mantel检验结果显示,土壤总氮(TN)、总磷(TP)、速效钾(AK)和硝态氮(NO_(3)^(-)-N)是驱动细菌群落的主要环境因子.通过研究高寒退化草地根际土壤细菌群落,可为因地制宜制定高寒退化草地管理措施提供理论依据,并为高寒草地退化的防治提供科学基础.Reseeding and fertilization are common ecological restoration measures in the Qinghai-Tibet Plateau Region for degraded grasslands,yet their impacts on rhizospheric bacterial communities remain unclear.This study utilized high-throughput sequencing technology to systematically investigate the characteristics of rhizospheric soil bacterial communities in degraded high-altitude grasslands managed with reseeding and fertilization.The results indicated a significant decrease in the Shannon and Simpson indices with fertilization treatment,while the reseeding+fertilization treatment exhibited the most pronounced impact on community structure.Actinomycetota,Pseudomonadota,and Acidobacteriota were the dominant phyla in the rhizosphere bacterial community.There was a decrease in network complexity and an increase in stability within bacterial communities with the reseeding,fertilization,and fertilization+reseeding treatments.The networks were predominantly characterized by positive correlations after fertilization,reseeding,and fertilization+reseeding treatments,involving key species like Pseudomonadota,Actinomycetota,and Acidobacteriota.Mantel tests revealed that soil total nitrogen(TN),total phosphorus(TP),available potassium(AK),and nitrate nitrogen(NO_(3)^(-)-N)were the key environmental factors in driving bacterial community composition.The study of the rhizosphere soil bacterial community in alpine degraded grassland can provide a theoretical basis for tailored management strategies in the restoration of degraded high-altitude grasslands and lays a scientific foundation for their conservation.

关 键 词：退化高寒草地 根际细菌群落 分子生态网络 补播 施肥 

分 类 号：X172[环境科学与工程—环境科学]