乌鲁木齐市孕妇血清中氨基酸水平与子痫前期的相关性研究  

Correlation between serum amino acid levels and preeclampsia in pregnant women in Urumqi

作　　者：朱静潆 陈琪 迟晓兰 杨苹靖文 冯子奕 薛淑媛 ZHU Jingying;CHEN Qi;CHI Xiaolan;YANG Pingjingwen;FENG Ziyi;XUE Shuyuan(Prenatal Diagnosis Center,Urumqi Maternal and Child Healthcare Hospital,Urumqi,Xinjiang 830000,China;Xinjiang Clinical Medical Research Center for Perinatal Diseases,Urumqi,Xinjiang 830000,China;School of Medicine,Shihezi University,Shihezi,Xinjiang 832000,China)

机构地区：[1]乌鲁木齐市妇幼保健院产前诊断中心,新疆乌鲁木齐830000 [2]新疆围产期疾病临床医学研究中心,新疆乌鲁木齐830000 [3]石河子大学医学院,新疆石河子832000

出　　处：《国际检验医学杂志》2024年第23期2883-2888,共6页International Journal of Laboratory Medicine

基　　金：新疆维吾尔自治区自然科学基金青年基金项目(2021D01B16);科技创新团队(天山创新团队)项目(2022TSYCTD0016)。

摘　　要：目的分析乌鲁木齐市子痫前期(PE)与正常妊娠孕妇血清中氨基酸水平的差异,并探讨差异有统计学意义的氨基酸与PE严重程度的相关性及其对PE患者的诊断价值。方法选取2021年6至2023年6月在乌鲁木齐市妇幼保健院就诊的49例正常妊娠孕妇作为对照组和55例PE孕妇作为PE组,PE组进一步分为轻度PE组(25例)和重度PE组(30例)。采用液相色谱-串联质谱技术测定孕妇血清中25种代谢物水平。采用Spearman相关性分析氨基酸与PE严重程度的相关性,采用Logistic回归分析对潜在混杂因素进行校正,采用受试者工作特征曲线分析差异有统计学意义的氨基酸对PE的诊断效能。结果PE组与对照组体质量指数(BMI)和分娩方式比较差异有统计学意义(P<0.05)。PE组血清组氨酸、苏氨酸、谷氨酰胺、乙醇胺、甲基组氨酸、犬尿氨酸水平高于对照组(P<0.05);重度PE组血清苏氨酸、谷氨酰胺、甲基组氨酸、犬尿氨酸水平高于轻度PE组和对照组(P<0.05);轻度PE组、重度PE组血清乙醇胺水平均高于对照组(P<0.05);重度PE组血清组氨酸水平高于对照组(P<0.05)。相关性分析结果显示,血清中谷氨酰胺、乙醇胺、甲基组氨酸、犬尿氨酸与PE的严重程度呈正相关(P<0.05);校正BMI后PE组和对照组苏氨酸、谷氨酰胺、甲基组氨酸水平差异有统计学意义(P<0.05);血清苏氨酸、谷氨酰胺和甲基组氨酸联合检测诊断PE的曲线下面积为0.734。结论血清苏氨酸、谷氨酰胺和甲基组氨酸水平与PE严重程度具有一定的相关性,且联合检测对PE具有较高的诊断价值。Objective To analyze the differences in serum amino acid levels between preeclampsia(PE) and normal pregnant women in Urumqi,and to explore the correlation between amino acids with statistically significant differences and PE severity,as well as their diagnostic value for PE patients.Methods A total of 49 normal pregnant women who visited Urumqi Maternal and Child Health Hospital from June 2021 to June 2023 were selected as the control group and 55 PE pregnant women were selected as the PE group,and the PE group was further divided into mild PE group(25 cases) and severe PE group(30 cases).Liquid chromatography-tandem mass spectrometry technology was used to determine the levels of 25 metabolites in pregnant women′s serum.Spearman correlation analysis was used to analyze the correlation between the amino acid levels and PE severity.Logistic regression was used to correct for potential confounding factors,and receiver operating characteristic curve was used to analyze the diagnostic efficacy of amino acids with significant differences in PE.Results There were statistically significant differences in the body mass index(BMI) and delivery method between the PE group and the control group(P<0.05).The serum levels of histidine,threonine,glutamine,ethanolamine,methylhistidine,and canine uric acid in the PE group were higher than those in the control group(P<0.05).The serum levels of threonine,glutamine,methylhistidine,and canine uric acid in the severe PE group were higher than those in the mild PE group and control group(P<0.05).The serum ethanolamine levels in the mild group and severe PE group were higher than those in the control group(P<0.05).The serum histidine levels in the severe PE group were higher than those in the control group(P<0.05).Correlation analysis showed that serum glutamine,ethanolamine,methylhistidine,and canine uric acid were positively correlated with the severity of PE(P<0.05).After adjusting for BMI,there were still statistically significant differences in the levels of threonine,glutamine,

关 键 词：子痫前期 氨基酸 液相色谱-串联质谱 代谢组学 

分 类 号：R446.1[医药卫生—诊断学]