蛋清源抗氧化肽对H_(2)O_(2)诱导的HEK293细胞的转录组学研究  

Transcriptomic Analyses of Antioxidant Peptides from Egg White on H_(2)O_(2)-Induced Oxidative Stress Injury in Human Embryonic Kidney 293

作　　者：张燕 魏汝君 张婷 刘博群 刘静波 Zhang Yan;Wei Rujun;Zhang Ting;Liu Boqun;Liu Jingbo(College of Food Science and Engineering,Jilin University,Changchun 130062)

机构地区：[1]吉林大学食品科学与工程学院,长春130062

出　　处：《中国食品学报》2024年第10期47-61,共15页Journal of Chinese Institute Of Food Science and Technology

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20201017KJ)。

摘　　要：通过转录组学及其生物信息学分析,研究蛋清源抗氧化肽WNWAD对H_(2)O_(2)诱导的HEK293细胞差异表达基因及相关信号转导通路。结果表明,损伤组与对照组相比,共有866条基因差异表达,保护组与损伤组相比,共有226条基因差异表达,其中95条基因在H_(2)O_(2)诱导损伤组(H_(2)O_(2)alone)和蛋清源抗氧化肽保护组(WNWAD+H_(2)O_(2))共同表达,占所有差异表达基因的9.4%。在这些基因中,41条基因在过氧化氢诱导损伤的HEK293中表达上调,然而,加入蛋清源抗氧化肽WNWAD预处理的HEK293细胞中这些基因显著下调;47条基因在过氧化氢诱导损伤的HEK293中表达下调,而加入蛋清源抗氧化肽WNWAD预处理的HEK293细胞中这些基因显著上调。生物信息学分析结果表明:对损伤组和保护组共有差异基因进行GO功能分析,结果显示,这些差异表达的基因可能参与细胞增殖、分化、凋亡以及细胞信号传导等多个生物学过程,其功能与细胞周期和细胞凋亡有较大的相关性。KEGG通路富集分析结果显示,由过氧化氢诱导损伤的HEK293细胞的差异表达的基因可能参与丝裂原活化蛋白(MAPK)信号传导途径、转化生长因子信号通路、WNT信号通路、hippo信号通路、肌醇磷酸盐代谢、p53信号通路、癌症调控通路等多条通路的传导,这些通路与氧化应激和细胞凋亡有较大的相关性。其中,13个差异基因参与MAPK信号通路的传导。This article used transcriptomic analysis and bioinformatics analysis to study the differentially expressed genes at the transcriptional level and related signal transduction pathways of antioxidant peptides from egg white on H_(2)O_(2)-induced oxidative stress injury in human embryonic kidney 293.The results showed that when HEK293 cells were injured by H_(2)O_(2),866 genes were differentially expressed compared with the control group.When egg white-derived active peptides were added,a total of 226 genes were differentially expressed in the protected group compared to the injured group.Among these genes,95 genes are expressed in the H_(2)O_(2)-induced injury group(H_(2)O_(2)alone)and the egg white-derived antioxidant peptide protection group(WNWAD+H_(2)O_(2)).Among these genes,41 genes were significantly up-regulated(≥2 fold)in HEK293 damaged by hydrogen peroxide,but these genes were significantly down-regulated in HEK293 cells pretreated with WNWAD,an antioxidant peptide derived from egg white;and 47 genes were significantly down-regulated(≤-2 fold)in HEK293 damaged by hydrogen peroxide,but these genes were significantly up-regulated in HEK293 cells pretreated with the anti-oxidant peptide.In the end,these differentially expressed genes were analyzed for GO function and KEGG pathway enrichment.The results showed that these differentially expressed genes may be involved in multiple biological processes such as cell proliferation,differentiation,apoptosis,and cell signal transduction.KEGG pathway enrichment analysis results show that the differentially expressed genes in HEK293 cells injured by hydrogen peroxide may be involved in the mitogen-activated protein(MAPK)signaling pathway,transforming growth factor signaling pathway,WNT signaling pathway,and hippo signaling pathway,inositol phosphate metabolism,p53 signaling pathway,cancer regulation pathway and many other pathways,these pathways have a greater correlation with oxidative stress and apoptosis.Among them,13 differential genes are involved in the cond

关 键 词：蛋清源抗氧化肽 氧化应激 转录组学 HEK293细胞 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]