小麦蛋白源5种活性肽的抗氧化作用及分子机制  

Antioxidant Effect and Molecular Mechanism of Five Active Peptides Derived from Wheat Protein

作　　者：刘文颖 任杰 吴晗硕 韩鲁佳[1] 谷瑞增 Liu Wenying;Ren Jie;Wu Hanshuo;Han Lujia;Gu Ruizeng(College of Engineering,China Agricultural University,Beijing 100083;Beijing Engineering Research Center of Protein and Functional Peptides,China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing 100015)

机构地区：[1]中国农业大学工学院,北京100083 [2]中国食品发酵工业研究院有限公司、北京市蛋白功能肽工程技术研究中,北京100015

出　　处：《中国食品学报》2024年第10期62-70,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：宁夏回族自治区重点研发计划项目(2021BEG02027)。

摘　　要：为了探讨小麦蛋白源活性肽的抗氧化作用及分子机制,从小麦蛋白中分离鉴定出5种活性肽:Leu-Tyr(LY)、Pro-Tyr(PY)、Tyr-Gln(YQ)、Ala-Pro-Ser-Tyr(APSY)、Arg-Gly-Gly-Tyr(RGGY)。通过DPPH自由基清除能力、羟基自由基清除能力、氧自由基吸收能力(ORAC值)、还原能力和总抗氧化能力(FRAP法)5种抗氧化评价指标研究其抗氧化作用。结果表明:LY、PY、YQ、APSY和RGGY均具有良好的抗氧化活性,其中PY的抗氧化能力最为突出,活性值分别为(88.90±2.25)%,(5143.68±65.45)μmol Trolox/g,(55.64±1.12)%,0.27±0.02和(0.68±0.01)mmol/L。采用量子化学方法对小麦蛋白源活性肽的化学结构进行分子模拟,获得稳定的优势构象,其分子能量分别为-2792298.02,-2490523.05,-2831549.24,-3977343.70,-4111743.04 kJ/mol。采用分子对接技术获得5种活性肽与DPPH的最优对接构型,LY和RGGY通过氢键和Π-阳离子静电相互作用与DPPH结合,PY通过Π-Π堆积、Π-阳离子静电相互作用和Π-阴离子静电相互作用与DPPH结合,YQ通过Π-阳离子静电相互作用和Π-阴离子静电相互作用与DPPH结合,APSY通过氢键、Π-Π堆积、Π-阳离子静电相互作用和Π-阴离子静电相互作用与DPPH结合。In order to explore the antioxidant effect and molecular mechanism of five active peptides derived from wheat protein,five active peptides[Leu-Tyr(LY),Pro-Tyr(PY),Tyr-Gln(YQ),Ala-Pro-Ser-Tyr(APSY),Arg-Gly-Gly-Tyr(RGGY)]were isolated and identified from wheat protein.Five antioxidant evaluation systems including DPPH free radical scavenging capacity,hydroxyl free radical scavenging capacity,oxygen free radical absorption capacity(ORAC value),reducing capacity and total antioxidant capacity(FRAP method)were used to investigate their antioxidant effects.The results showed that LY,PY,YQ,APSY and RGGY had good antioxidant activity in five evaluation systems,among which PY had the most prominent antioxidant activity,and the activity values were(88.90±2.25)%,(5143.68±65.45)μmol Trolox/g,(55.64%±1.12)%,0.27±0.02 and(0.68±0.01)mmol/L,respectively.The chemical structure of wheat protein-derived active peptides was simulated by quantum chemistry,and stable dominant conformation was obtained.The molecular energies were-2792298.02,-2490523.05,-2831549.24,-3977343.70 and-4111743.04 kJ/mol.The optimal docking configurations of five active peptides and DPPH were obtained by molecular docking technology.LY and RGGY bound to DPPH through hydrogen bonds andΠ-cationic electrostatic interaction,PY bound to DPPH throughΠ-ΠaccumulationΠ-cationic electrostatic interaction andΠ-anionic electrostatic interaction,YQ bound to DPPH throughΠ-cationic electrostatic interaction andΠ-qnionic electrostatic interaction,and APSY bound to DPPH through hydrogen bond,Π-Πaccumulation,Π-cationic electrostatic interaction andΠ-anionic electrostatic interaction.

关 键 词：小麦蛋白源肽 抗氧化活性 分子机制 量子化学计算 分子对接技术 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]