植物乳植杆菌CCFM8661对小鼠免疫功能及肠道菌群的影响  

Effect of Lactiplantibacillus plantarum CCFM8661 on immune function and intestinal florainmice

作　　者：张芮 刘铭洋 李柏良 ZHANG Rui;LIU Mingyang;LI Bailiang(College of Food,Northeast Agricultural University,Harbin 150036,China)

机构地区：[1]东北农业大学食品学院,哈尔滨150036

出　　处：《中国乳品工业》2024年第11期5-12,共8页China Dairy Industry

基　　金：国家自然科学基金面上项目(32072190);中国博士后科学基金面上项目(2022M721071)。

摘　　要：文章研究了植物乳植杆菌(Lactiplantibacillus plantarum)CCFM8661对环磷酰胺(Cyclophosphamide,CTX)免疫抑制小鼠免疫功能及肠道菌群的调节效果。将60只BALB/c小鼠随机分为5组:对照组、模型组、CCFM8661低剂量组(2×10^(6)CFU)、CCFM8661中剂量组(2×10^(7)CFU)、CCFM8661高剂量组(2×10^(8)CFU),通过连续30d测定小鼠胸腺指数、溶血空斑数、脾脏指数、脾淋巴细胞增殖能力、足跖肿胀程度、半数溶血值、肝脾巨噬细胞吞噬能力、腹腔巨噬细胞吞噬率、NK细胞活性、T淋巴细胞亚群和肠道菌群评价植物乳植杆菌CCFM8661对免疫抑制小鼠免疫功能的改善作用。结果表明,植物乳植杆菌CCFM8661可以显著增强小鼠免疫功能并调节小鼠肠道菌群的结构与丰度。研究为开发具有免疫调节作用的保健食品提供理论依据。This study investigated the regulatory effects of Lactiplantibacllus plantarum CCFM8661 on immune function and intestinal microbiota in cyclophosphamide-induced immunosuppressed mice.Sixty BALB/c mice were randomly divided into 5 groups:blank control,model control,low-dose CCFM8661(2×10^(6)CFU),medium-dose CCFM8661(2×10^(7)CFU),and high-dose CCFM8661(2×10^(8)CFU).The mice were treated for 30 days,and immune function was evaluated by measuring thymus index,hemolysis plaque assay,spleen index,splenic lymphocyte proliferation,paw swelling degree,half hemolysis value,phagocytic capacity of liver and spleen macrophages,phagocytic rate of peritoneal macrophages,NK cell activity,T lymphocyte subsets,and intestinal microbiota.The results showed that Lactiplantibacllus plantarum CCFM8661 significantly enhanced immune function in mice and regulated the intestinal microbiota.It was concluded that Lactiplantibacllus plantarum CCFM8661 has a significant immune-enhancing effect,providing a theoretical basis for the development of immune-modulating health foods.

关 键 词：植物乳植杆菌CCFM8661 免疫抑制 免疫调节 肠道菌群 

分 类 号：TS201.3[轻工技术与工程—食品科学]