婴儿肠道源长双歧杆菌B2-01的益生特性及其高密度培养  

Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01,a Strain Isolated from the Intestinal Tract of Infants

作　　者：彭芸燕 钟舒莹 刘冬梅 许喜林[1] PENG Yunyan;ZHONG Shuying;LIU Dongmei;XU Xilin(School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510641

出　　处：《食品科学》2025年第1期28-39,共12页Food Science

基　　金：广州市科技计划资助项目(201903010015);广东省乡村振兴战略专项资金种业振兴项目(2022-440000-4301030402-9565);国家自然科学基金面上项目(31771908)。

摘　　要：以广州市3月龄健康婴儿粪便中分离出的乳酸杆菌为研究对象,经分子生物学鉴定确认为长双歧杆菌(Bifidobacterium longum),命名为B2-01。对B2-01进行包括体外抗氧化活性和肝损伤保护活性在内的益生特性和高密度培养研究,结果表明:B2-01具有较强的1,1-二苯基-2-三硝基苯肼自由基、羟自由基、超氧阴离子自由基、2,2′-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力和抗脂质过氧化能力;在最高作用水平(DMEM完全培养基中发酵上清液体积分数10%)下,过氧化氢损伤和乙醇损伤L02细胞存活率可分别提高至89.10%和91.38%,同时能显著降低肝细胞中转氨酶的活力;高密度培养研究优化后的最佳培养条件确定为酵母浸提粉添加量3.45%、葡萄糖2.48%、乳糖2.79%、细菌学蛋白胨3.00%、低聚半乳糖2.50%。在此优化条件下,发酵液的活菌数最高可达4.20×10^(9) CFU/m L,比优化前(7.71×10^(8) CFU/mL)提高5.45倍。In this study,a Lactobacillus strain was isolated from the feces of healthy 3-month-old infants in Guangzhou,identified and named as Bifidobacterium longum B2-01 by molecular biology,and its probiotic properties,such as in vitro antioxidant activity and hepatoprotective activity,and high-density culture were investigated.The results showed that B2-01 had a strong ability to scavenge 1,1-diphenyl-2-picrylhydrazyl(DPPH),hydroxyl,superoxide anion,and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radicals and to resist lipid peroxidation.At the highest concentration of 10%(V/V)in the fermentation supernatant of DMEM medium,strain B2-01 increased the survival rates of L02 cells against hydrogen peroxide damage and alcohol damage to 89.10%and 91.38%,respectively,while significantly reducing the transaminase activity in hepatocytes.The optimal conditions for high-density culture were determined as follows:yeast extract concentration 3.45%,glucose concentration 2.48%,lactose concentration 2.79%,bacteriological peptone concentration 3%,and oligogalactose concentration 2.5%.Under these conditions,the maximum viable bacterial count of 4.20×10^(9) CFU/mL was observed in the fermentation broth,which was 6.45 times as high as that before optimization(7.71×10^(8) CFU/mL).

关 键 词：长双歧杆菌 婴儿肠道 抗氧化活性 酒精肝保护 高密度培养 

分 类 号：Q93[生物学—微生物学]