儿茶素减轻小鼠肺泡巨噬细胞炎症反应的作用及机制研究  

Study on the anti-inflammatory effect and mechanism of catechin on mouse alveolar macrophages

作　　者：陈淑珍 许秋凤 王皇斌 黄莎莎 黄丽端 熊闽 CHEN Shuzhen;XU Qiufeng;WANG Huangbin;HUANG Shasha;HUANG Liduan;XIONG Min(School of Public Health,Fujian Medical University,Fuzhou 350122,China;Fujian Provincial Key Laboratory of Functional and Clinical Translational Medicine,Department of Basic Medicine,Xiamen Medical College,Xiamen 361023,China;Department of Pharmacy,Xiamen Medical College,Xiamen 361023,China)

机构地区：[1]福建医科大学公共卫生学院,福州350122 [2]厦门医学院基础医学部机能与临床转化福建省高校重点实验室,厦门361023 [3]厦门医学院药学系,厦门361023

出　　处：《生物学杂志》2024年第6期26-30,共5页Journal of Biology

基　　金：福建省自然科学基金面上项目(2021J01343);厦门医学院科技计划项目(K2023-24)。

摘　　要：研究旨在探讨比较儿茶素和表儿茶素在小鼠肺泡巨噬细胞(MH-S)中的抗炎作用及其机制。利用脂多糖(LPS)刺激MH-S细胞建立炎症模型,用MTS法和LDH法检测儿茶素和表儿茶素对MH-S细胞活力的影响;ELISA法检测细胞上清液IL-1β、IL-6和TNF-α含量;qRT-PCR法检测细胞Nos2、Il-6、Tnf-α、Grp78、Ire1α、Perk和Atf6 mRNA表达;Western Blot法检测iNOS蛋白表达。结果显示,6.25~100μg/mL儿茶素和表儿茶素无显著细胞毒性,可浓度依赖地下调LPS诱导的IL-1β分泌和iNOS表达,还降低iNOS、IL-6以及内质网应激(ERS)相关蛋白IRE1α的mRNA转录水平。低浓度儿茶素的抗炎活性强于表儿茶素,高浓度则相反。研究表明,儿茶素和表儿茶素可以通过减少IL-1β、IL-6分泌,抑制iNOS蛋白表达发挥抗炎作用,其抗炎作用可能与抑制ERS有关。The purpose of this study was to explore and compare the anti-inflammatory effects and the possible mechanisms of catechin and epicatechin in mouse alveolar macrophages MH-S.Lipopolysaccharide LPS was used to stimulate MH-S cells to establish a cellular inflammation model.The effects of catechin and epicatechin on MH-S cell viability were detected by MTS and LDH assays.The contents of interleukin-1βIL-1β,IL-6,and TNF-αin the supernatant were detected by enzyme-linked immunosorbent assay ELISA.Real-time quantitative polymerase chain reaction qRT-PCR was used to detect the mRNA transcription levels of Nos2,Il-6 Tnf-α,Grp78 Ire1αPerk,and Atf6.The protein expression of iNOS was detected by Western Blot.The results showed that 6.25-100μg/mL of catechin and epicatechin had no significant toxicity on MH-S cells.Both catechin and epicatechin down-regulated the secretion of IL-1βand the protein expression of iNOS in LPS-induced MH-S cells in a concentration-dependent manner.Meanwhile,catechin and epicatechin reduced the mRNA transcription levels of iNOS,IL-6 and endoplasmic reticulum stress ERS-related protein IRE1α.The anti-inflammatory activity of catechin was stronger than that of epicatechin at low concentrations,but catechin was lower than epicatechin at high concentrations.These results indicated that catechin and epicatechin had anti-inflammatory effects by reducing the secretion of IL-1β,IL-6 and the expression of iNOS,which might be related to the inhibition of ERS.

关 键 词：儿茶素 表儿茶素 MH-S细胞 炎症 内质网应激 

分 类 号：Q26[生物学—细胞生物学] R967[医药卫生—药理学]